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BMAA extraction of cyanobacteria samples: which method to choose?
β-N-Methylamino-l-alanine (BMAA), a neurotoxin reportedly produced by cyanobacteria, diatoms and dinoflagellates, is proposed to be linked to the development of neurological diseases. BMAA has been found in aquat...
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Article
Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China
The diazotrophic communities in a rice paddy field were characterized by a molecular polyphasic approach including DNA/RNA-DGGE fingerprinting, real time RT-PCR analysis of nifH gene and the measurement of nitrog...
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Article
Improving derivatization efficiency of BMAA utilizing AccQ-Tag® in a complex cyanobacterial matrix
Two different assays have been developed and used in order to investigate the optimal conditions for derivatization and detection of acid β-N-methyl-amino-l-alanine (BMAA) in a cyanobacterial sample. BMAA was ext...
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Article
Old women with a recent fall history show improved muscle strength and function sustained for six months after finishing training
Background and aims: Restricted physical activity as a consequence of chronic disease or injury is a predictor of functional decline. The aim of this study was to test the hypothesis that a 6-mon...
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Article
The first protein map of Synechococcus sp. strain PCC 7942
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Article
The first protein map of Synechococcus sp. strain PCC 7942
The first protein map was developed of Synechococcus sp. strain PCC 7942, a model organism for studies of photosynthesis, prokaryotic circadian rhythms, cell division, carbon-concentrating mechanisms, and adaptiv...
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Article
Phylogeny of symbiotic cyanobacteria within the genus Nostoc based on 16S rDNA sequence analyses
A phylogenetic analysis of selected symbiotic Nostoc strain sequences and available database 16S rDNA sequences of both symbiotic and free-living cyanobacteria was carried out using maximum likelihood and Bayesia...
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Book
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Cyanobacterial Diversity and Specificity in Plant Symbioses
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Article
Characterization by genotypic methods of symbiotic Nostoc strains isolated from five species of Gunnera
The genetic diversity of ten symbiotic Nostoc strains isolated from different Gunnera species was investigated. The strains were analyzed using molecular methods with different taxonomic resolutions, including r...
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Article
Cyanobacterial diversity in geographically related and distant host plants of the genus Gunnera
The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short ...
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Article
Cloning and characterization of a pathogen-induced chitinase in Brassica napus
A chitinase cDNA clone from rapeseed (Brassica napus L. ssp. Oleifera) was isolated. The cDNA clone, ChB4, represents a previously purified and characterized basic chitinase isozyme. The longest open reading fram...
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Article
Induction and purification of chitinase in Brassica napus L. ssp. oleifera infected with Phoma lingam
A pathogen-induced chitinase (EC 3.2.1.14) was isolated from cotyledons of oilseed rape (Brassica napus cv. Bienvenu) 8 d after inoculation with Phoma lingam. The purified chitinase has a molecular weight of 30 k...
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Article
Immunogold localization of chymotrypsin inhibitor-2, a lysine-rich protein, in develo** barley endosperm
Chymotrypsin inhibitor-2, a lysine-rich protein in the barley endosperm, has been localized at the ultrastructural level by immunocytochemistry in develo** barley endosperm cells 14 days post anthesis. The p...
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Article
Monoclonal antibodies to hordein polypeptides
Monoclonal antibodies to hordein polypeptides have been produced and characterized. All antibodies reacted with more than one polypeptide, indicating that different hordein polypeptides have common epitopes. O...
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Article
Immunological screening for specific protein content in barley seeds
A screening method employing immunofluorescence has been developed to judge the amount of specific polypeptides, such as the high lysine protein chymotrypsin inhibitor 2 (CI-2) in the endosperm of barley.
