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Article
Chemo-enzymatic synthesis of a glycosylated peptide containing a complex N-glycan based on unprotected oligosaccharides by using DMT-MM and Endo-M
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycop...
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Chapter
Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)
Carbohydrate-protein interaction is very important in understanding the function of sugar chains. The immobilization or fluorescence modification of oligosaccharides was investigated to understand the biologic...
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Chapter
Chemo-Enzymatic Synthesis of Glycoconjugates
Synthesis of glycoconjugate is one of the most important difficulties in carbohydrate chemistry. Although many chemical synthetic methods have been developed, the synthesis of glycoconjugates with natural glyc...
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Article
A chemical synthesis of UDP-LacNAc and its regioisomer for finding ‘oligosaccharide transferases’
A chemical synthesis of uridine 5’-diphospho-N-acetyllactosamine (Galβ(1→4)GlcNAc-UDP; UDP-LacNAc) and Galβ(1→3)GlcNAc-UDP is described. Coupling of the disaccharide imidate derivatives with dibenzylphosphate gav...
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Article
Chemo-enzymatic synthesis and structure-activity study of artificially N-glycosylated eel calcitonin derivatives with a complex type oligosaccharide
Starting from N-glycosylated eel calcitonin derivatives that contain an N-acetyl-D-glucosamine residue specifically at the 3rd, 14th, 20th or 26th amino acid residue, corresponding glycopeptides with a complex-ty...
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Chapter
Chemo-enzymatic syntheses of eel calcitonin analogs having natural N-linked oligosaccharides
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Article
Effect of carbohydrate structure on biological activity of artificially N-glycosylated eel calcitonin
To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensio...