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Article
Open AccessRecombinant therapeutic proteins degradation and overcoming strategies in CHO cells
Mammalian cell lines are frequently used as the preferred host cells for producing recombinant therapeutic proteins (RTPs) having post-translational modified modification similar to those observed in proteins ...
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Article
CMV/AAT promoter of MAR-based episomal vector enhanced transgene expression in human hepatic cells
We have previously developed a non-viral episomal vector based on matrix attachment region (MAR) that can facilitate plasmid replication episomally in mammal cells. In this study, we have focused on the develo...
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Article
Progress in fed-batch culture for recombinant protein production in CHO cells
Nearly 80% of the approved human therapeutic antibodies are produced by Chinese Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant protein, fed-batch culture is typically used ...
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Chapter and Conference Paper
Comparative Study on Applicability of Prestack AVO Inversion and Prestack Elastic Parameter Inversion for Reservoir Prediction
Prestack AVO inversion and prestack elastic parameter inversion are the common technical methods for reservoir prediction. Finding the direction to improve the reliability of inversion results and selecting t...
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Article
Progress of cationic gene delivery reagents for non-viral vector
Gene delivery systems play a vital role in gene therapy and recombinant protein production. The advantages of using gene delivery reagents for non-viral vector include the capacity to accommodate a large packa...
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Article
Construction of an expression vector mediated by the dual promoter for prokaryotic and mammalian cell expression system
The aim of this study was to construct an expression vector mediated by the dual promoter that can simultaneously drive the recombinant protein production in eukaryotic and prokaryotic cells. The prokaryotic T...
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Article
Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems
Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeut...
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Article
Distance effect characteristic of the matrix attachment region increases recombinant protein expression in Chinese hamster ovary cells
Previously, we have found that the matrix attachment region (MAR) may confer a ‘distance effect’ on transgene expression. This work aims to systematically explore the increased transgene expression in transfec...
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Article
Effects of different 2A peptides on transgene expression mediated by tricistronic vectors in transfected CHO cells
Multicistronic vectors can increase transgene expression and decrease the imbalance of gene expression in the Chinese hamster ovary (CHO) cell expression system. Small, self-cleaving 2A peptides have a high cl...
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Article
Human genome-derived TOP1 matrix attachment region enhances transgene expression in the transfected CHO cells
To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) ce...
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Article
Construction strategies for develo** expression vectors for recombinant monoclonal antibody production in CHO cells
Recent years have seen the use of recombinant proteins in the treatment of different diseases. Among them, monoclonal antibodies (mAbs) are currently the fastest growing class of bio-therapeutic recombinant pr...
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Article
Open AccessRetraction Note: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells
This paper has been retracted at the request of the authors.
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Article
Open AccessSequence architecture and sedimentary characteristics of a Middle Jurassic incised valley, western Sichuan depression, China
The Middle Jurassic Shaximiao Formation encompasses tens of meters of thick lowstand meandering valley (LMV) strata in the western Sichuan foreland basin. Ancient LMVs newly discovered in this area were furthe...
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Article
Open AccessHuman rhinovirus internal ribosome entry site element enhances transgene expression in transfected CHO-S cells
Chinese hamster ovary (CHO) cells are mainly used for recombinant protein production. However, the unstable transgene expression and lower transgene copy numbers are the major issues need to be resolved. Here,...
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Protocol
A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue
Most traditional post-electrophoretic processes need several hours to several days to finish the whole staining process and traditional staining solutions all contain methanol, acetic acid, or phosphoric acid,...
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Article
Open AccessRETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells
In the present study, six commonly used promoters, including cytomegalovirus major immediate-early (CMV), the CMV enhancer fused to the chicken beta-actin promoter (CAG), human elongation factor-1α (HEF-1α), m...
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Article
Open AccessMatrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells
Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell expression system. To investigate the effects of MAR combinations on transgene expressio...
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Article
Open AccessImpact of Different Promoters on Episomal Vectors Harbouring Characteristic Motifs of Matrix Attachment Regions
We previously demonstrated that the characteristic sequence of matrix attachment regions (MARs) allows transgenes to be maintained episomally in CHO cells. In the present study, six commonly used promoters fro...
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Article
Open AccessTreating Colon Cancer Cells with FK228 Reveals a Link between Histone Lysine Acetylation and Extensive Changes in the Cellular Proteome
The therapeutic value of FK228 as a cancer treatment option is well known and various types of cancer have been shown to respond to this drug. However, the complete mechanism of FK228 and the affect it has on ...
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Article
The effect of recombinant lentiviral vector encoding miR-145 on human esophageal cancer cells
miR-145, a newly identified microRNA molecule, is hypothesized to function as a tumor suppressor, but this activity has not been investigated in esophageal l carcinoma (EC). The aim of this study was to invest...
