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Article
Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells
The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic recons...
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Article
Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12iMax
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system is continually optimized to achieve the most efficient gene editing effect. The Cas12iMax, a Cas12i varia...
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Article
Open AccessThree-dimensional surface motion capture of multiple freely moving pigs using MAMMAL
Understandings of the three-dimensional social behaviors of freely moving large-size mammals are valuable for both agriculture and life science, yet challenging due to occlusions in close interactions. Althoug...
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Article
Open AccessGeneration and characterization of stable pig pregastrulation epiblast stem cell lines
Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model development and livestock breeding. Despite ongoing attempts since the 1990s, ...
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Article
Open AccessGeneration of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver
Mice with humanized livers are important models to study drug toxicology testing, development of hepatitis virus treatments, and hepatocyte transplantation therapy. However, the huge difference between mouse a...
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Article
Open AccessEstablishment of a humanized swine model for COVID-19
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Article
Open AccessDomesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs
Blastocyst complementation by pluripotent stem cell (PSC) injection is believed to be the most promising method to generate xenogeneic organs. However, ethical issues prevent the study of human chimeras in the...
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Article
Open AccessA novel porcine model reproduces human oculocutaneous albinism type II
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Article
Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs
Insulin-like growth factor 2 (IGF2) is an important growth factor, which promotes growth and development in mammals during fetal and postnatal stages. Using CRISPR–Cas9 system, we generated multiple founder pigs ...
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Article
Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis
Human Waardenburg syndrome 2A (WS2A) is a dominant hearing loss (HL) syndrome caused by mutations in the microphthalmia-associated transcription factor (MITF) gene. In mouse models with MITF mutations, WS2A is tr...
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Article
Open AccessOne-step generation of triple gene-targeted pigs using CRISPR/Cas9 system
Pig shows multiple superior characteristics in anatomy, physiology, and genome that have made this species to be more suitable models for human diseases, especially for neurodegenerative diseases, because they...
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Article
Cell therapy in diabetes: current progress and future prospects
Diabetes mellitus, characterized by the impaired metabolism of insulin secretion in β cells, is becoming one of the most prevalent diseases around the world. Recently, cell replacement based on differentiation...
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Article
Open AccessEfficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs
Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-gu...
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Article
Open AccessEfficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs
Pigs are ideal organ donors for xenotransplantation and an excellent model for studying human diseases, such as neurodegenerative disease. Transcription activator-like effector nucleases (TALENs) are used wide...
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Article
Open AccessEfficient generation of mouse ESCs-like pig induced pluripotent stem cells
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Article
Open AccessEpigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor—m-carboxycinnamic acid bishydroxamide (CBHA)
Insufficient epigenetic reprogramming of donor nuclei is believed to be one of the most important causes of low development efficiency of mammalian somatic cell nuclear transfer (SCNT). Previous studies have s...
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Article
Open AccessOne-step generation of knockout pigs by zygote injection of CRISPR/Cas system
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Article
Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells
Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently...
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Article
Viable Fertile Mice Generated from Fully Pluripotent iPS Cells Derived from Adult Somatic Cells
Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, ...
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Article
Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells