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Open AccessA new randomization procedure based on multiple covariates and applicable to parallel studies with simultaneous enrollment of all subjects prior to intervention
Parallel intervention studies involving volunteers usually require a procedure to allocate the subjects to study-arms. Statistical models to evaluate the different outcomes of the study-arms will include study...
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Article
Open AccessMeasuring health promotion: translating science into policy
Commonly, it is the end of life when our health is deteriorating, that many will make drastic lifestyle changes to improve their quality of life. However, it is increasingly recognized that bringing good healt...
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Article
Open AccessMulti-parameter comparison of a standardized mixed meal tolerance test in healthy and type 2 diabetic subjects: the PhenFlex challenge
A key feature of metabolic health is the ability to adapt upon dietary perturbations. Recently, it was shown that metabolic challenge tests in combination with the new generation biomarkers allow the simultane...
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Open AccessThe impact of micronutrient status on health: correlation network analysis to understand the role of micronutrients in metabolic-inflammatory processes regulating homeostasis and phenotypic flexibility
Vitamins and carotenoids are key micronutrients facilitating the maintenance of health, as evidenced by the increased risk of disease with low intake. Optimal phenotypic flexibility, i.e., the ability to respo...
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Article
The insulin resistance phenotype (muscle or liver) interacts with the type of diet to determine changes in disposition index after 2 years of intervention: the CORDIOPREV-DIAB randomised clinical trial
The aim of the study was to determine whether basal insulin resistance (IR) phenotype (muscle and/or liver) determines the effect of long-term consumption of a Mediterranean diet or a low-fat diet on tissue-sp...
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Open AccessPhenotypic flexibility as a measure of health: the optimal nutritional stress response test
Nutrition research is struggling to demonstrate beneficial health effects, since nutritional effects are often subtle and long term. Health has been redefined as the ability of our body to cope with daily-life...
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Article
Analyzing metabolomics-based challenge tests
Challenge tests are used to assess the resilience of human beings to perturbations by analyzing responses to detect functional abnormalities. Well known examples are allergy tests and glucose tolerance tests. ...
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Open AccessAssessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges
Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress res...
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Article
Open AccessControlling false discovery rates in factorial experiments with between-subjects and within-subjects tests
The False Discovery Rate (FDR) controls the expected number of false positives among the positive test results. It is not straightforward how to conduct a FDR controlling procedure in experiments with a factor...
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Article
Semi-targeted metabolomic approaches to validate potential markers of health for micronutrients: analytical perspectives
Recommended dietary allowances for micronutrients fluctuate noticeably within European Union countries. The Network of Excellence EURRECA (EURopean micronutrient RECommendations Aligned) aims at harmonising mi...
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Open AccessPlasma metabolomics and proteomics profiling after a postprandial challenge reveal subtle diet effects on human metabolic status
We introduce the metabolomics and proteomics based Postprandial Challenge Test (PCT) to quantify the postprandial response of multiple metabolic processes in humans in a standardized manner. The PCT comprised ...
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Article
Open AccessVisualization and identification of health space, based on personalized molecular phenotype and treatment response to relevant underlying biological processes
Being able to visualize multivariate biological treatment effects can be insightful. However the axes in visualizations are often solely defined by variation and thus have no biological meaning. This makes the...
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Article
Metabolomics of prolonged fasting in humans reveals new catabolic markers
Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human “fasting metabolome” obtained from analysis of plasm...
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Open AccessThe Micronutrient Genomics Project: a community-driven knowledge base for micronutrient research
Micronutrients influence multiple metabolic pathways including oxidative and inflammatory processes. Optimum micronutrient supply is important for the maintenance of homeostasis in metabolism and, ultimately, ...
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Article
Open AccessChallenges of molecular nutrition research 6: the nutritional phenotype database to store, share and evaluate nutritional systems biology studies
The challenge of modern nutrition and health research is to identify food-based strategies promoting life-long optimal health and well-being. This research is complex because it exploits a multitude of bioacti...
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Open AccessInsight in modulation of inflammation in response to diclofenac intervention: a human intervention study
Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce ...
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Article
Open AccessMass-spectrometry-based metabolomics: limitations and recommendations for future progress with particular focus on nutrition research
Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characteriz...
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Open AccessImproving the analysis of designed studies by combining statistical modelling with study design information
In the fields of life sciences, so-called designed studies are used for studying complex biological systems. The data derived from these studies comply with a study design aimed at generating relevant informat...
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CDG: a new case of a combined defect in the biosynthesis of N- and O-glycans
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Article
MBD1, MBD2 and CGBP genes at chromosome 18q21 are infrequently mutated in human colon and lung cancers
The genes MBD1 and MBD2 encode methyl-CpG binding proteins that suppress transcription from methylated promoters. In contrast, CGBP encodes a protein that binds promoters containing unmethylated CpG and stimulate...
