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Article
Open AccessCorrection to: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
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Article
Open AccessQuality control of protein reagents for the improvement of research data reproducibility
Proteins and peptides are amongst the most widely used research reagents but often their quality is inadequate and can result in poor data reproducibility. Here we propose a simple set of guidelines that, when...
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Article
Correction to: Macromolecular interactions in vitro, comparing classical and novel approaches
A correction to this paper has been published: https://doi.org/10.1007/s00249-021-01540-6
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Article
Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale survey
As the scientific community strives to make published results more transparent and reliable, it has become obvious that poor data reproducibility can often be attributed to insufficient quality control of expe...
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Article
Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments
NanoTemper Monolith instruments have gained enormous popularity for measuring molecular interactions both in academia and industry. The underlying technology has been extensively reviewed along with its assump...
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Article
Macromolecular interactions in vitro, comparing classical and novel approaches
Biophysical quantification of protein interactions is central to unveil the molecular mechanisms of cellular processes. Researchers can choose from a wide panel of biophysical methods that quantify molecular i...
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Article
Open AccessReproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolec...
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Protocol
Assessing and Improving Protein Sample Quality
One essential prerequisite of any experiment involving a purified protein, such as interaction studies or structural and biophysical characterization, is to work with a “good-quality” sample in order to ensure...
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Chapter
Optimale Steuerung des Zusammenspiels von Verbrennungs- und Elektromotor
Mit dem Einsatz verbrennungsmotorisch angetriebener Fahrzeuge sind mehrere Ziele verbunden. Aus ökologischer Sicht ist ein geringer Ausstoß an gesundheitsschädlichen Abgasen sowie an Kohlenstoffdioxid, welches...
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Chapter
The Transporters Associated with Antigen Processing (TAP)
Class I molecules of the major histocompatibility complex (MHC) present peptides derived from endogenous proteins at the cell surface. During viral infection or malignant transformation a different set of pept...
