-
Chapter
Microbial Degradation of Chemical Pesticides and Bioremediation of Pesticide-Contaminated Sites in China
The intensive use of chemical pesticides has also resulted in serious environmental problems. Biodegradation of chemical pesticides and bioremediation of pesticide-contaminated sites by microorganisms is consi...
-
Article
Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides
To identify and characterize a novel aryloxyphenoxypropionate (AOPP) herbicide-hydrolyzing carboxylesterase from Aquamicrobium sp. FPB-1.
-
Article
Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil
A bacteria strain, YW6, capable of utilizing monocrotophos (MCP) as the sole carbon and nitrogen sources for growth was isolated from paddy soil and identified as Starkeya novella. Strain YW6 completely degraded ...
-
Article
Reductive dehalogenation of 3,5-dibromo-4-hydroxybenzoate by an aerobic strain of Delftia sp. EOB-17
To confirm the reductive dehalogenation ability of the aerobic strain of Delftia sp. EOB-17, finding more evidences to support the hypothesis that reductive dehalogenation may occur extensively in aerobic bacteri...
-
Article
Salinicola zeshunii sp. nov., a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm
The taxonomic status of a moderately halophilic bacterium, strain N4T, isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic a...
-
Article
Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil
One rhamnolipid-producing bacterial strain named Pseudomonas aeruginosa BSFD5 was isolated and characterized. Its rhlABRI cassette including necessary genes for rhamnolipid synthesis was cloned and transformed in...
-
Article
Characterization of a Newly Isolated Highly Effective 3,5,6-Trichloro-2-pyridinol Degrading Strain Cupriavidus pauculus P2
A bacterial strain P2 capable of degrading 3,5,6-trichloro-2-pyridinol (TCP) was isolated and characterized. Phylogenetic analysis based on 16S rRNA gene sequence indicated that it belonged to the genus of Cupria...
-
Article
Characterization of the nitrobenzene-degrading strain Pseudomonas sp. a3 and use of its immobilized cells in the treatment of mixed aromatics wastewater
A bacterial strain Pseudomonas sp. a3 capable of degrading nitrobenzene, phenol, aniline, and other aromatics was isolated and characterized. When nitrobenzene was degraded, the release of NH4 + w...
-
Article
An isofenphos-methyl hydrolase (Imh) capable of hydrolyzing the P–O–Z moiety of organophosphorus pesticides containing an aryl or heterocyclic group
Organophosphorus pesticide (OP) hydrolases play key roles in the degradation and decontamination of agricultural and household OPs and in the detoxification of chemical warfare agents. In this study, an isofen...
-
Article
Cloning and characterization of a novel amidase from Paracoccus sp. M-1, showing aryl acylamidase and acyl transferase activities
A novel amidase gene, designated pamh, was cloned from Paracoccus sp. M-1. Site-directed mutagenesis and bioinformatic analysis showed that the PamH protein belonged to the amidase signature enzyme family. PamH w...
-
Article
Recent Advances in the Biodegradation of Chlorothalonil
Chlorothalonil (TPN; 2,4,5,6-tetrachloroisophthalonitrile) has been widely used as a broad-spectrum chlorinated aromatic fungicide and its application resulted in global pollution commonly detected in the dive...
-
Article
Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by Ancylobacter sp. XJ-412-1 Isolated from Soil
Ancylobacter sp. XJ-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. When metsulfuron-methyl was provided as the sole carbon source, more than 90.5% of metsulfuron...
-
Article
Identification of the Biochemical Degradation Pathway of Triazophos and its Intermediate in Diaphorobacter sp. TPD-1
Triazophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A bacterial strain, Diaphorobacter sp. TPD-1, capable of using triazophos and its intermediate, 1-phe...
-
Article
Hydrolytic Dechlorination of Chlorothalonil by Ochrobactrum sp. CTN-11 Isolated from a Chlorothalonil-Contaminated Soil
A bacterial strain, designated as CTN-11, capable of degrading chlorothalonil (CTN), was isolated from a chlorothalonil-contaminated soil in China. Based on the morphological, biochemical characteristics and c...
-
Article
Degradation of methamidophos by Hyphomicrobium species MAP-1 and the biochemical degradation pathway
Methamidophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A facultative methylotroph, Hyphomicrobium sp. MAP-1, capable of high efficiently degrading metham...
-
Article
Expression of Methyl Parathion Hydrolase in Pichia pastoris
In the Pichia pastoris expression system, increasing the copy number of the expression cassette often has the effect of increasing the amount of protein expressed. To improve the expression level of methyl parath...
-
Article
Isolation, Characterization of a Strain Capable of Degrading Imazethapyr and Its Use in Degradation of the Herbicide in Soil
One strain of bacterium IM-4, capable of degrading imazethapyr (IMZT), was isolated from the IMZT-contaminated soil. The isolate was identified as Pseudomonas sp. according to its physiological characteristics, b...
-
Article
Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, south China
In order to understand the community structure of diazotrophs in red soil and effects of organic manure Application on the structure, four nifH gene libraries were constructed: the control (CK), low manure (LM), ...
-
Article
Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon
A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into...
-
Article
A gene linB2 responsible for the conversion of β-HCH and 2,3,4,5,6-pentachlorocyclohexanol in Sphingomonas sp. BHC-A
Commercial formulations of hexachlorocyclohexane (HCH) consist of a mixture of four isomers: α, β, γ, and δ. All four isomers are toxic and recalcitrant pollutants. β-HCH is more problematic due to its longer ...
