Abstract
One rhamnolipid-producing bacterial strain named Pseudomonas aeruginosa BSFD5 was isolated and characterized. Its rhlABRI cassette including necessary genes for rhamnolipid synthesis was cloned and transformed into the chromosome of P. putida KT2440 by a new random transposon vector without introducing antibiotic-resistance marker, generating a genetically engineered microorganism named P. putida KT2440-rhlABRI, which could stably express the rhlABRI cassette and produce rhamnolipid at a yield of 1.68 g l−1. In experiments using natural soil, it was shown that P. putida KT2440-rhlABRI could increase the dissolution of pyrene and thus promote its degradation by indigenous microorganisms. P. putida KT2440-rhlABRI thus demonstrated potential for enhancing the remediation of soils contaminated with polycyclic aromatic hydrocarbons.
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Acknowledgments
This work was supported by Chinese National Natural Science Fund (31070099, 30830001 and 31000060), Natural Science Foundation of Jiangsu Province, China (BK2009312), the Opening Fund of State Key Laboratory of Soil and Sustainable Agriculture (Y052010025) and the Major Technology Special Social Development Project (2007C13059).
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Cao, L., Wang, Q., Zhang, J. et al. Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil. World J Microbiol Biotechnol 28, 2783–2790 (2012). https://doi.org/10.1007/s11274-012-1088-0
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DOI: https://doi.org/10.1007/s11274-012-1088-0