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Article
Novel silicatein-like protein for biosilica production from Amphimedon queenslandica and its use in osteogenic composite fabrication
Efforts to find sustainable and eco-friendly ways to conduct chemical reactions have led to the mimicking of nature. In this study, a new silica polymerization protein that can produce silica in an environment...
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Article
Anti-cancer Effect of Hyoscyamus muticus Extract via Its Activation of Fas/FasL-ASK1-p38 Pathway
Hyoscyamus muticus L. is a traditional medicine used as antispasmodic and sedative. Herein, we aimed to determine the phytochemical constituents and for the first time its anti-cancer activities. The phytochemica...
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Article
Osteostimulating Ability of β-tricalcium Phosphate/collagen Composite as a Practical Bone-grafting Substitute: In vitro and in vivo Comparison Study with Commercial One
A biocompatible and biodegradable bioceramic, β-tricalcium phosphate (β-TCP) has been widely considered as a safe and effective alternative to autograft for bone reconstruction due to its excellent osteoconduc...
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Article
Open AccessBiosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration
Bioceramic β-tricalcium phosphate (β-TCP) is used as a bone-grafting material and a therapeutic drug carrier for treatment of bone defects in the oral and maxillofacial regions due to the osteoconductivity and...
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Article
Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel
Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity ...
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Article
Production of TiO2-deposited Diatoms and Their Applications for Photo-catalytic Degradation of Aqueous Pollutants
Biogenic TiO2 diatoms can be prepared by metabolic incorporation of TiO2 on the frustule surface of the diatoms. To utilize the photo-catalytic activity of biogenic TiO2 for practical applications, high productio...
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Article
Solvo-hydrothermal synthesis of calcium phosphate nanostructures from calcium inositol hexakisphosphate precursor in water-ethanol mixed solutions
We report the synthesis and characterization of crystalline calcium phosphate (CaP) nanostructures from calcium inositol hexakisphosphate (CaIP6) precursor in water-ethanol mixed solutions. We show how these CaPs...
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Article
Open AccessA systematic study of hexavalent chromium adsorption and removal from aqueous environments using chemically functionalized amorphous and mesoporous silica nanoparticles
We report on the synthesis and characterization of highly monodisperse amorphous silica nanoparticles (ASNs) and mesoporous silica nanoparticles (MSNs) with particle sizes of 15–60 nm. We demonstrate adsorptio...
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Article
Fusion tags to enhance heterologous protein expression
Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of proteins, insufficient yield, and inclusion body formation. N...
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Article
The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli
The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of...
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Article
Anticancer effect of nor-wogonin (5, 7, 8-trihydroxyflavone) on human triple-negative breast cancer cells via downregulation of TAK1, NF-κB, and STAT3
Nor-wogonin, a polyhydroxy flavone, has been shown to possess antitumor activity. However, the mechanisms responsible for its antitumor activity are poorly studied. Herein, we investigated the mechanisms of no...
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Article
Improvement in the Reproducibility of a Paper-based Analytical Device (PAD) Using Stable Covalent Binding between Proteins and Cellulose Paper
Paper-based analytical devices (PADs) have been widely used in many fields because they are affordable and portable. For reproducible quantitative analysis, it is crucial to strongly immobilize proteins on PAD...
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Article
Chemical characterization of dissolved organic matter in moist acidic tussock tundra soil using ultra-high resolution 15T FT-ICR mass spectrometry
Global warming is considered one of the most serious environmental issues, substantially mediating abrupt climate changes, and has stronger impacts in the Arctic ecosystems than in any other regions. In partic...
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Article
Expression and characterization of a codon-optimized alkaline-stable carbonic anhydrase from Aliivibrio salmonicida for CO2 sequestration applications
The CO2 mineralization process, accelerated by carbonic anhydrase (CA) was proposed for the efficient capture and storage of CO2, the accumulation of which in the atmosphere is the main cause of global warming. H...
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Article
A cold-adapted tyrosinase with an abnormally high monophenolase/diphenolase activity ratio originating from the marine archaeon Candidatus Nitrosopumilus koreensis
To obtain an acidic and cold-active tyrosinase, which potentially minimizes unwanted self-oxidation of tyrosinase-catalyzed catechols, including 3,4-dihydroxyphenylalanine at elevated pH and high temperature.
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Article
The role of extracellular polymeric substances in reducing copper inhibition to nitrification in activated sludge
This study presents the role of extracellular polymeric substances (EPS) in reducing the inhibitory effects of copper towards nitrifying activated sludge. EPS could ameliorate copper toxicity in activated slud...
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Article
Recombinant production of a shell matrix protein in Escherichia coli and its application to the biomimetic synthesis of spherulitic calcite crystals
To overcome the limited production capability of shell matrix proteins and efficiently conduct in vitro CaCO3 biomineralization studies, a putative recombinant shell matrix protein was prepared and characterized.
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Article
Characterization of Phosphoenolpyruvate Carboxylase from Oceanimonas smirnovii in Escherichia coli
In this study, phosphoenolpyruvate carboxylase (PEPC) derived from Oceanimonas smirnovii (OS) was expressed as a soluble protein in Escherichia coli BL21(DE3). We isolated OS-PEPC (a recombinant PEPC protein) by ...
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Article
Diverse Influences of Androgen-Disrupting Chemicals on Immune Responses Mounted by Macrophages
Androgen-disrupting chemicals (ADCs) can alter male sexual development. Although the effects of ADCs on hormone disruption have been studied, their influence on the immune response is not fully understood. To ...
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Article
High activity and stability of codon-optimized phosphoenolpyruvate carboxylase from Photobacterium profundum SS9 at low temperatures and its application for in vitro production of oxaloacetate
Phosphoenolpyruvate carboxylase (PEPC) of Photobacterium profundum SS9 can be expressed and purified using the Escherichia coli expression system. In this study, a codon-optimized PEPC gene (OPPP) was used to inc...
