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19 Result(s)
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Seth Blackshaw
Biomedicine
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Article
Open AccessElevated nuclear TDP-43 induces constitutive exon skip**
Cytoplasmic inclusions and loss of nuclear TDP-43 are key pathological features found in several neurodegenerative disorders, suggesting both gain- and loss-of-function mechanisms of disease. To study gain-of-...
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Article
Open AccessAmyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes
Amongst risk alleles associated with late-onset Alzheimer’s disease (AD), those that converged on the regulation of microglia activity have emerged as central to disease progression. Yet, how canonical amyloid...
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Book
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Chapter
Winding the Clock: Development of Hypothalamic Structures Controlling Biological Timing and Sleep
The daily cycle of sleep and wake governs all aspects of behavior and is under tight homeostatic regulation. The core circadian oscillator in turn restricts sleep to specific intervals of the solar day. The ne...
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Article
Open AccessReactive microglia and IL1β/IL-1R1-signaling mediate neuroprotection in excitotoxin-damaged mouse retina
Microglia and inflammation have context-specific impacts upon neuronal survival in different models of central nervous system (CNS) disease. Herein, we investigate how inflammatory mediators, including microgl...
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Protocol
Antibody Specificity Profiling Using Protein Microarrays
Antibodies are the most widely used reagent for isolation and detection of specific proteins. However, using antibodies that are not highly specific in these studies can generate inaccurate and misleading data...
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Protocol
In Vivo Electroporation of Develo** Mouse Retina
In vivo electroporation enables the transformation of retinal tissue with engineered DNA plasmids, facilitating the selective expression of desired gene products. This method achieves plasmid transfer via the ...
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Chapter
Regulation of Body Weight and Metabolism by Tanycyte-Derived Neurogenesis in Young Adult Mice
The hypothalamus controls many homeostatic and instinctive physiological processes, including the sleep-wake cycle, food intake, and sexually dimorphic behaviors. These behaviors are regulated by environmental...
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Protocol
Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Develo** Mouse Diencephalon
The mammalian diencephalon gives rise to the thalamus and hypothalamus. The thalamus contains multiple sensory nuclei and relays sensory information to corresponding cortical areas, while the hypothalamus is a...
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Article
Open AccessEstrogen-related receptor β deficiency alters body composition and response to restraint stress
Estrogen-related receptors (ERRs) are orphan nuclear hormone receptors expressed in metabolically active tissues and modulate numerous homeostatic processes. ERRs do not bind the ligand estrogen, but they are ...
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Article
Open AccessErratum to: Heme Oxygenase-2 Is Neuroprotective in Cerebral Ischemia
Sylvain Doré, Kenji Sampei, Shozo Goto, Nabil J Alkayed, Daniel Guastella, Seth Blackshaw, Michela Gallagher, Richard J Traystman, Patricia D Hurn, Raymond C Koehler, and Solomon H Snyder. (1999) Heme Oxygenas...
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Protocol
High-Throughput RNA In Situ Hybridization in Mouse Retina
The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. T...
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Article
Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche
The authors describe a neurogenic niche in the postnatal hypothalamus of mice wherein β2-tanycytes generate neurons in response to high-fat diet. Blocking this neurogenesis leads to attenuated weight gain and ...
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Article
Open AccessThe long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity
Thousands of different long non-coding RNAs are expressed during embryonic development, but the function of these molecules remains largely unexplored.
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Article
Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation
The mouse retina includes cone photoreceptors optimized for short (S) and medium (M) wavelengths; the difference lies in the expression of S and M opsin. Onishi and colleagues find that, in M cones, thyroid ho...
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Article
A genomic atlas of mouse hypothalamic development
This Resource chronicles dynamic gene expression patterns in the develo** hypothalamus from embryonic day 10.5 through maturity. The authors find that Shh must be expressed in the hypothalamic basal plate fo...
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Article
Stem cells that know their place
Human neural stem cells could be used to treat many diseases, but a major problem with this approach is that these cells give rise to few neurons in the brain. Now a new priming method increases production of ...
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Article
Mutations in a new photoreceptor-pineal gene on 17p cause Leber congenital amaurosis
Leber congenital amaurosis (LCA, MIM 204000) accounts for at least 5% of all inherited retinal disease1 and is the most severe inherited retinopathy with the earliest age of onset2. Individuals affected with LCA ...
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Article
Heme Oxygenase-2 Is Neuroprotective in Cerebral Ischemia
Heme oxygenase (HO) is believed to be a potent antioxidant enzyme in the nervous system; it degrades heme from heme-containing proteins, giving rise to carbon monoxide, iron, and biliverdin, which is rapidly r...
