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Protocol
Nanopore Sequencing Techniques: A Comparison of the MinKNOW and the Alignator Sequencers
Whole genome and transcriptome analyses represent powerful tools. Despite improvements in sequencing methodology, whole transcriptome analyses are still tedious, especially for methodologies producing long rea...
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Article
Open AccessSyntaxin 18 regulates the DNA damage response and epithelial-to-mesenchymal transition to promote radiation resistance of lung cancer
Radiotherapy is an important modality in lung cancer treatment. Despite advances in treatment planning and dose delivery, patient benefit is still limited by in-field relapse and metastatic recurrence. Simulta...
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Article
Oncometabolites suppress DNA repair by disrupting local chromatin signalling
Deregulation of metabolism and disruption of genome integrity are hallmarks of cancer1. Increased levels of the metabolites 2-hydroxyglutarate, succinate and fumarate occur in human malignancies owing to somatic ...
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Article
Mitochondrial DNA stress signalling protects the nuclear genome
The mammalian genome comprises nuclear DNA (nDNA) derived from both parents and mitochondrial DNA (mtDNA) that is maternally inherited and encodes essential proteins required for oxidative phosphorylation. Tho...
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Article
Open AccessHigh-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
DNA- and histone-related research frequently comprises the quantitative analysis of protein modifications, such as histone phosphorylation. Analysis of accumulation and disappearance of protein foci are used t...
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Article
Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair
The hereditary cancer syndromes hereditary leiomyomatosis and renal cell cancer (HLRCC) and succinate dehydrogenase–related hereditary paraganglioma and pheochromocytoma (SDH PGL/PCC) are linked to germline lo...
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Article
Open AccessThe Focinator - a new open-source tool for high-throughput foci evaluation of DNA damage
The quantitative analysis of foci plays an important role in many cell biological methods such as counting of colonies or cells, organelles or vesicles, or the number of protein complexes. In radiation biology...
