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Article
Deciphering the regulatory networks involved in mild and severe salt stress responses in the roots of wild grapevine Vitis vinifera spp. sylvestris
Transcriptional regulatory networks are pivotal components of plant’s response to salt stress. However, plant adaptation strategies varied as a function of stress intensity, which is mainly modulated by climat...
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Article
Evidence of an active role of resveratrol derivatives in the tolerance of wild grapevines (Vitis vinifera ssp. sylvestris) to salinity
Resveratrol and its derivatives are the most important phytoalexins with a crucial role in plant defense mechanisms. These compounds can occur either naturally or in response to abiotic stresses. Among them, s...
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Article
Ectopic expression of a grapevine alkaline α-galactosidase seed imbibition protein VvSIP enhanced salinity tolerance in transgenic tobacco plants
Alpha-galactosidase seed imbibition protein (VvSIP) isolated from Vitis vinifera is up-regulated upon salt stress and mediates osmotic stress responses in a tolerant grapevine cultivar. So far, little is known ab...
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Article
Recent advances in biotechnological studies on wild grapevines as valuable resistance sources for smart viticulture
Cultivated grapevines, Vitis vinifera subsp. sativa, are thought to have been domesticated from wild populations of Vitis vinifera subsp. sylvestris in Central Asia. V. vinifera subsp. sativa is one of the most e...
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Article
A Grapevine-Inducible Gene Vv-α-gal/SIP Confers Salt and Desiccation Tolerance in Escherichia coli and Tobacco at Germinative Stage
Grapevine is an important fruit crop cultivated worldwide. Previously, we have reported the characterization of a salt stress-inducible gene Vv-α-gal/SIP isolated from the tolerant grapevine cultivar Razegui. In ...
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Article
Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress
The expression of a gene originally identified by means of a salt-stress-associated partial cDNA showing a genotype-dependent response, was studied upon exposure to salt and drought stress and during seed deve...
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Article
Short-term response of wild grapevines (Vitis vinifera L. ssp. sylvestris) to NaCl salinity exposure: changes of some physiological and molecular characteristics
The physiological and molecular response to salt stress was studied in two wild grapevine (Vitis vinifera L. ssp. sylvestris or Vitis sylvestris) accessions “Khédhayria” and “Houamdia”, previously identified as s...
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Article
Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress
The expression of a gene originally identified by means of a salt stress-associated partial cDNA showing a genotype dependent response was studied upon exposure to salt and drought stress and during seed devel...
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Article
Proteomic and Transcriptomic Analysis of Grapevine PR10 Expression During Salt Stress and Functional Characterization in Yeast
Proteomic and transcriptomic analyses of pathogenesis-related proteins, PR10, were carried out by subjecting adult plants of the salt-tolerant grapevine cv. Razegui to 100 mM NaCl for 6 and 24 h under controll...
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Article
Improvement of an RNA purification method for grapevine (Vitis vinifera L.) suitable for cDNA library construction
A method is described, which consistently yields high quality total RNA from grapevine. Dissolving of crude RNA pellets in borate-containing buffer, instead of normally used water before a selective lithium ch...
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Chapter and Conference Paper
Expression analysis of salt stress responsive genes in grapevines
In Tunisia, highly tolerant autochthonous varieties represent a valuable resource for elucidating mechanisms of plant adaptation to salinity. Our aim is to determine which genes in these Tunisian grapevine var...
