-
Article
Trabeculectomy training in England: are we safe at training? Two year surgical outcomes
To define the safety profile of trainee trabeculectomy surgery in the United Kingdom. Surgical exposure for trainees in England is limited due to service requirements, the European working time directive const...
-
Chapter and Conference Paper
Determination of Wall Strain in Model Cerebral Aneurysms by Image Registration
The rupture of intracranial aneurysms often has devastating consequences, and it is important to be able to identify those aneurysms that are at risk. One potential marker for imminent rupture might be strain ...
-
Article
Correction of the disease phenotype in the mouse model of Stargardt disease by lentiviral gene therapy
Autosomal recessive Stargardt disease (STGD1) is a macular dystrophy caused by mutations in the ABCA4 (ABCR) gene. The disease phenotype that is most recognized in STGD1 patients, and also in the Abca4−/− mouse (...
-
Article
Argumentation and Primary Science
The importance of argumentation in science education is outlined and the relatively low level of argumentation typically observed in classrooms in the UK is noted, along with possible reasons for this. The res...
-
Article
EIAV vector-mediated delivery of endostatin or angiostatin inhibits angiogenesis and vascular hyperpermeability in experimental CNV
We evaluated the efficacy of equine infectious anaemia virus (EIAV)-based lentiviral vectors encoding endostatin (EIAV.endostatin) or angiostatin (EIAV.angiostatin) in inhibiting angiogenesis and vascular hype...
-
Article
Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue
Lymphoid follicles cluster in the terminal rectum of various animal species and of man and hence this site may be important in the development of immune responses to pathogens. For the induction of immune resp...
-
Article
Hypoxia-regulated transgene expression in experimental retinal and choroidal neovascularization
Recombinant AAV vectors mediate efficient and sustained transgene expression in retinal tissues and offer a powerful approach to the local, sustained delivery of angiostatic proteins for the treatment of ocula...
-
Article
Hypoxia-mediated tumour targeting
Hypoxia is a common physiological feature of tumours. It activates a signalling cascade that culminates in the stabilization of the HIF-1 transcription factor and activation of genes that possess a hypoxia res...
-
Article
Evaluating knowledge in acute illness: Critical Care Educational Project
-
Chapter and Conference Paper
A Randomised Trial of Two Radiotherapy Schedules in the Adjuvant Treatment of Stage I Seminoma (MRC TE18) — Preliminary Report
Adjuvant post-orchidectomy radiotherapy cures the majority of patients with stage I seminoma, but as approximately 80 per cent would remain relapse-free on surveillance alone, minimising radiotherapy — and hen...
-
Article
The macrophage – a novel system to deliver gene therapy to pathological hypoxia
The use of activated macrophages in the treatment of cancer has been largely ineffectual. By ‘arming’ these cells with the ability to express a therapeutic gene we demonstrate significant advances in the effic...
-
Article
The Macrophage-Exploiting Hypoxia With Cell Based Delivery of GDEPT
-
Article
Preclinical Evaluation of Human Cytochrome P450 2b6 (Cyp2b6) as a Therapeutic Gene Delivered by a Retroviral Vehicle for Gene-Directed Enzyme Prodrug Therapy (GDEPT) of Cancer
-
Article
A Physiologically Regulated Adenoviral Vector for the Treatment of Ischaemic Disease
-
Article
An adenoviral vector regulated by hypoxia for the treatment of ischaemic disease and cancer
Recombinant adenoviral vectors have a number of advantages for gene therapy, including transduction of a range of dividing and non-dividing cell types. However, this broad range may be a disadvantage if non-ta...
-
Chapter
Eosinophilia-Myalgia Syndrome Case-Associated Contaminants in Commercially Available 5-Hydroxytryptophan
Recently, 5-hydroxy-L-tryptophan (5-OHTrp) has been promoted as an alternative to banned L-tryptophan as a dietary supplement. It has been claimed to help alleviate obesity, insomnia, depression, and headaches...
-
Chapter
Structural Characterization of Contaminants in Commercial Preparations of Melatonin by on-Line Hplc-Electrospray Ionization-Tandem Mass Spectrometry
Three different commercially available melatonin preparations were analyzed by on-line HPLC-electrospray ionization-tandem mass spectrometry. All three samples contained the same impurities at the ∼0.1-0.5% le...
-
Chapter
Structural Characterization of Case-Associated Contaminants Peak C and Ff in L-Tryptophan Implicated in Eosinophilia-Myalgia Syndrome
We have characterized the structures of two case-associated contaminants of the Showa Denko L-tryptophan known to cause eosinophilia-myalgia syndrome (EMS). A combination of on-line accurate mass HPLC-electros...
-
Article
Identifying sites of protein modification by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and on-line membrane preconcentration-capillary electrophoresis-tandem mass spectrometry
A strategy for rapidly identifying the number and sites of chemical or posttranslational modification of proteins is described. The use of matrix-assisted laser desorption/ionization-time-of-flight-mass spectr...
-
Article
Characterization of N-benzylcarbazole and its metabolites from microsomal mixtures by tandem mass spectrometry
The metabolism of N-benzylcarbazole (NBC) was studied in vitro using hamster hapetic microsomes to establish whether the corresponding amide is formed. This work was carried out in order to see if the extremel...
