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Article
Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia
Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm−2) for 1, 3, 5 and 7 s before cu...
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Article
Somatic embryogenesis inCicer arietinum L: Influence of genotype and auxins
Plant regeneration through somatic embryogenesis was obtained in chickpea (Cicer arietinum L.) using immature cotyledons and immature embryonal axes as explants. 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3....
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Article
Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.)
Somatic embryos were induced from immature cotyledons and immature embryonal axis ofArachis hypogaea L. on L-6 basal medium supplemented with NAA, picloram or 2,4-D at 5–50 mg 1-1. Immature embryonal axis produce...
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Article
Isolation of sodium chloride tolerant cell lines and plants in finger millet
Sodium chloride tolerant cell lines of finger millet were isolated from embryogenic cultures growing on MS medium supplemented with picloram (2 mg I-1), kinetin (0.1 mg l-1) and sodium chloride (1 %) at the end o...
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Chapter
Regeneration and Differentiation of Protoplasts in Grain Legumes
Somatic hybridization following protoplast fusion is a reliable tool to combine genotypes which are difficult to cross sexually Since the first report of sustained division and regeneration of plants from prot...
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Chapter
Transformation Studies in Tobacco, Moth Bean and Brassica Species
Besides the Aqrobacterium tumefaciens mediated gene transfer, the direct gene transfer is an established technique for transforming single cells of higher plants. Transformation rates are influenced by factors li...
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Article
Stable transformation of moth bean Vigna aconitifolia via direct gene transfer
Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts trea...
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Chapter
Regeneration of Wheat, Rye and Triticale Plants Through Tissue Culture
Recent developments in plant cell cultures have fostered the hope that this technique could be of invaluable help in crop improvement (1, 2). However, for effective utilization of this technique, methods have ...