Abstract
Besides the Aqrobacterium tumefaciens mediated gene transfer, the direct gene transfer is an established technique for transforming single cells of higher plants. Transformation rates are influenced by factors like heat shock, PEG-concentration, carrier-DNA and incubation time (Shillito et al. 1985). Here, the influence of plant genotype on the transformation rates was investigated using several varieties of four plant species. The importance of plant genotype on the regeneration potential in cell and tissue culture is recognized since many years. Other important factors, incubation media and vectors carrying different antibiotic marker genes and/or promotors, also influenced the transformation rates obtained.
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© 1988 Kluwer Academic Publishers
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Köhler, F., Afsar, K., Golz, C., Eapen, S., Schieder, O. (1988). Transformation Studies in Tobacco, Moth Bean and Brassica Species. In: Puite, K.J., Dons, J.J.M., Huizing, H.J., Kool, A.J., Koornneef, M., Krens, F.A. (eds) Progress in Plant Protoplast Research. Current Plant Science and Biotechnology in Agriculture, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2788-9_130
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DOI: https://doi.org/10.1007/978-94-009-2788-9_130
Publisher Name: Springer, Dordrecht
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