Abstract
Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts treated with PEG and plasmid DNA containing the coding region for aminoglycoside phosphotransferase gene (NPT II). The plant cultivar used was an important factor in attaining higher transformation frequencies. Transformation was confirmed by Southern blot analysis using a non-radioactive detection system. Attempts to transform mesophyll and suspension cultured cells by this method were unsuccessful. Protoplasts electroporated with the plasmid pCAP212, which codes for chloramphenicol acetyltransferase, exhibited transient expression of this gene two days after treatment while electroporated cells did not show this enzyme activity. It is therefore assumed that the DNA uptake is prevented by the cell wall.
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References
Binding H (1974) Z Pflanzenphysiol 74: 327–356
Fromm M, Taylor LP, Walbot V (1985) Proc Natl Acad Sci USA 82: 5824–5828
Garcia JA, Hill J, Goldbach R (1986) Plant Sci 44: 37–46
Golz C, Köhler F, Eapen S, Schieder O (1987) 7. Wissenschaftl. Tagung der Gesellschaft für Entwicklungsbiologie mit der deutschen Sektion der IAPTC, FU Berlin, Berlin, pp. 89
Hain R, Stabel P, Czernilofsky AP, Steinbiß HH, Herrera-Estrella L, Schell J (1985) Mol Gen Genet 199: 161–168
Krens FH, Molendijk L, Wullems GJ, Schilperoort RA (1982) Nature 296: 72–74
Lörz H, Baker B, Schell J (1985) Mol Gen Genet 199: 178–182
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor NY
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Neumann R, Rudloff P, Eggers HJ (1986) Naturwissenschaften 73: 553–555
Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) EMBO J 3: 2717–2722
Paszkowski J, Pisan B, Shillito RD, Hohn T, Hohn B, Potrykus I (1986) Plant Mol Biol 6: 303–312
Potrykus I, Paszkowski J, Saul MW, Petruska J Shillito RD (1985a) Mol Gen Genet 199: 169–177
Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985b) Mol Gen Genet 199: 183–188
Schieder O (1984) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, Vol 1, Academic Press, Orlando, pp 350–355
Shillito RD, Paszkowski J, Potrykus I (1983) Plant Cell Rep 2: 244–247
Shillito RD, Saul MW, Paszkowski J, Müller M, Potrykus I (1985) Bio/Technol 3: 1099–1103
Soberon X, Covarrabias L, Bolivar F (1980) Gene 9: 287–300
Southern EM (1975) J Mol Biol 98: 503–517
Velten J, Schell J (1985) Nucleic Acid Res 13: 6981–6998
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Köhler, F., Golz, C., Eapen, S. et al. Stable transformation of moth bean Vigna aconitifolia via direct gene transfer. Plant Cell Reports 6, 313–317 (1987). https://doi.org/10.1007/BF00272007
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DOI: https://doi.org/10.1007/BF00272007