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  1. No Access

    Article

    Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia

    Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm−2) for 1, 3, 5 and 7 s before cu...

    R. K. Jangid, R. Sharma, Y. Sudarsan, S. Eapen, G. Singh… in Biologia Plantarum (2010)

  2. No Access

    Article

    Somatic embryogenesis inCicer arietinum L: Influence of genotype and auxins

    Plant regeneration through somatic embryogenesis was obtained in chickpea (Cicer arietinum L.) using immature cotyledons and immature embryonal axes as explants. 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3....

    S. Eapen, L. George in Biologia Plantarum (1994)

  3. No Access

    Article

    Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.)

    Somatic embryos were induced from immature cotyledons and immature embryonal axis ofArachis hypogaea L. on L-6 basal medium supplemented with NAA, picloram or 2,4-D at 5–50 mg 1-1. Immature embryonal axis produce...

    S. Eapen, L. George, P. S. Rao in Biologia Plantarum (1993)

  4. No Access

    Article

    Isolation of sodium chloride tolerant cell lines and plants in finger millet

    Sodium chloride tolerant cell lines of finger millet were isolated from embryogenic cultures growing on MS medium supplemented with picloram (2 mg I-1), kinetin (0.1 mg l-1) and sodium chloride (1 %) at the end o...

    J. Pius, S. Eapen, L. George, P. S. Rao in Biologia Plantarum (1993)

  5. No Access

    Article

    Transformation of the grain legumeVigna aconitifolia Jacq Marechal byAgrobacterium tumefaciens, regeneration of shoots

    Hypocotyl, stem, leaf and shoot-apex of one-week-old seedlings ofVigna aconitifolia were inoculated with the nopaline wild type (T37) ofAgrobacterium tumefaciens to induce tumours. Hypocotyl segments of 1 cm leng...

    Ravinder Gill, S Eapen, P S Rao in Proceedings / Indian Academy of Sciences (1988)

  6. No Access

    Chapter

    Regeneration and Differentiation of Protoplasts in Grain Legumes

    Somatic hybridization following protoplast fusion is a reliable tool to combine genotypes which are difficult to cross sexually Since the first report of sustained division and regeneration of plants from prot...

    R. Gill, S. Eapen, P. S. Rao in Progress in Plant Protoplast Research (1988)

  7. No Access

    Chapter

    Transformation Studies in Tobacco, Moth Bean and Brassica Species

    Besides the Aqrobacterium tumefaciens mediated gene transfer, the direct gene transfer is an established technique for transforming single cells of higher plants. Transformation rates are influenced by factors li...

    F. Köhler, K. Afsar, C. Golz, S. Eapen… in Progress in Plant Protoplast Research (1988)

  8. No Access

    Article

    Cultivar dependence of transformation rates in moth bean after co-cultivation of protoplasts with Agrobacterium tumefaciens

    A simplified protoplast regeneration system for Vigna aconitifolia was developed. A plating efficiency of 60% was obtained using mesophyll protoplasts from 10-day-old seedlings. By co-cultivation of protoplasts w...

    S. Eapen, F. Köhler, M. Gerdemann, O. Schieder in Theoretical and Applied Genetics (1987)

  9. No Access

    Article

    Stable transformation of moth bean Vigna aconitifolia via direct gene transfer

    Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts trea...

    F. Köhler, C. Golz, S. Eapen, H. Kohn, O. Schieder in Plant Cell Reports (1987)

  10. No Access

    Article

    Callus induction from protoplasts of V. unguiculata, V. sublobata and V. mungo

    Protoplasts were isolated from hypocotyl of V. mungo (L.) Hepper or hypocotyl-derived callus of V. sublobata (Phaseolus sublobata Roxb.) and V. unguiculata (L.) Walp (syn. V. sinensis (L.) Saviex Hassk) using an ...

    R. Gill, S. Eapen, P. S. Rao in Theoretical and Applied Genetics (1987)

  11. No Access

    Article

    Regeneration of plants from cultured root explants of mothbean (Vigna aconitifolia L. Jacq. Marechal)

    Decapitated seedling root explants of seven cultivars of mothbean (Vigna aconitifolia) cultured on Murashige and Skoog's basal medium without any phytohormone gave rise to plantlets at the end of 4–5 weeks. Addit...

    S. Eapen, R. Gill in Theoretical and Applied Genetics (1986)

  12. No Access

    Chapter

    Regeneration of Wheat, Rye and Triticale Plants Through Tissue Culture

    Recent developments in plant cell cultures have fostered the hope that this technique could be of invaluable help in crop improvement (1, 2). However, for effective utilization of this technique, methods have ...

    S. Eapen, P. S. Rao in Plant Cell Culture in Crop Improvement (1983)

  13. No Access

    Article

    Two NAD-dependent alcohol dehydrogenases (E.C. 1.1.1.1) in callus cultures of wheat, rye and triticale

    Two NAD-dependent alcohol dehydrogenases ADH-1 and ADH-2, under independent genetic control of genes designated as Adh-1 and Adh-2 located on chromosomes 4A, 4B and 4D, have been reported in aestivum wheat (Hart ...

    K. N. Suseelan, C. R. Bhatia, S. Eapen in Theoretical and Applied Genetics (1982)