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Article
Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions
The endodermal germ layer gives rise to respiratory epithelium, hepatocytes, pancreatic cells and intestinal lineages, among other cell types. These lineages can be differentiated from human pluripotent stem c...
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Article
Open AccessBulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating...
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Article
Cardiac differentiation of human pluripotent stem cells in scalable suspension culture
This protocol describes how to generate large quantities of cardiomyocytes from human pluripotent stem cells using a bioreactor. Cells are differentiated by application of Wnt pathway modulators while growing ...
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Chapter
Induced Pluripotent Stem Cells Differentiate into Functional Cardiomyocytes
The conversion of somatic cells into so called induced pluripotent stem cells (iPSCs) is generally considered as major breakthrough in stem cell research, and cardiomyocytes derived from human iPS cells are co...
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Chapter and Conference Paper
A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells
Embryonic Stem (ES) cells are self- renewing and pluripotent cells that can differentiate into a variety of cell lineages. Implementation in tissue engineering or as model systems for drug discovery makes ES c...
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Article
Scalable expansion of human pluripotent stem cells in suspension culture
Routine commercial and clinical applications of human pluripotent stem cells (hPSCs) and their progenies will require increasing cell quantities that cannot be provided by conventional adherent culture technol...
