Page
%P
-
Article
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
A promising cell-therapy approach for heart failure aims at differentiating human pluripotent stem cells (hPSCs) into functional cardiomyocytes (CMs) in vitro to replace the disease-induced loss of patients’ h...
-
Article
Open AccessFabrication of heart tubes from iPSC derived cardiomyocytes and human fibrinogen by rotating mold technology
Due to its structural and functional complexity the heart imposes immense physical, physiological and electromechanical challenges on the engineering of a biological replacement. Therefore, to come closer to c...
-
Article
Open AccessCircular RNA circZFPM2 regulates cardiomyocyte hypertrophy and survival
Hypertrophic cardiomyopathy (HCM) constitutes the most common genetic cardiac disorder. However, current pharmacotherapeutics are mainly symptomatic and only partially address underlying molecular mechanisms. ...
-
Article
Open AccessMatrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation
Human pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by reliable, economically viable production processes. The suspension culture...
-
Article
Open AccessScalable production of tissue-like vascularized liver organoids from human PSCs
The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissue...
-
Article
Open AccessAirway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis
Idiopathic pulmonary fibrosis (IPF) is a fatal disease with limited treatment options. In this study, we focus on the properties of airway basal cells (ABC) obtained from patients with IPF (IPF-ABC). Single ce...
-
Article
Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors
Macrophages derived from human induced pluripotent stem cells (iPSCs) have the potential to enable the development of cell-based therapies for numerous disease conditions. We here provide a detailed protocol f...
-
Article
Generation of heart-forming organoids from human pluripotent stem cells
Heart-forming organoids (HFOs) derived from human pluripotent stem cells (hPSCs) are a complex, highly structured in vitro model of early heart, foregut and vasculature development. The model represents a pote...
-
Article
Open AccessPublisher Correction: Human heart-forming organoids recapitulate early heart and foregut development
-
Article
Open AccessHuman heart-forming organoids recapitulate early heart and foregut development
Organoid models of early tissue development have been produced for the intestine, brain, kidney and other organs, but similar approaches for the heart have been lacking. Here we generate complex, highly struct...
-
Chapter
Heart Muscle Tissue Engineering
The human heart is a complex, multicellular organ with limited regenerative capacity. In vitro modeling of the human heart is not only important to better understand the composition and function thereof, but also...
-
Article
Open AccessComparing human iPSC-cardiomyocytes versus HEK293T cells unveils disease-causing effects of Brugada mutation A735V of NaV1.5 sodium channels
Loss-of-function mutations of the SCN5A gene encoding for the sodium channel α-subunit NaV1.5 result in the autosomal dominant hereditary disease Brugada Syndrome (BrS) with a high risk of sudden cardiac death in...
-
Article
Open AccessFemtosecond laser-based nanosurgery reveals the endogenous regeneration of single Z-discs including physiological consequences for cardiomyocytes
A highly organized cytoskeleton architecture is the basis for continuous and controlled contraction in cardiomyocytes (CMs). Abnormalities in cytoskeletal elements, like the Z-disc, are linked to several disea...
-
Protocol
Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay
The ideal cell culture model should mimic the cell physiology and the mechanical and the chemical cues that are present in specific tissues and organs, within a convenient high-throughput format. A possible ke...
-
Protocol
Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors
This chapter describes a detailed protocol on human pluripotent stem cells (hPSCs) cultivation as matrix-free cell-only aggregates in defined and xeno-free culture medium in stirred tank bioreactors (STBRs). S...
-
Protocol
Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies
Cardiomyocytes from human pluripotent stem cells (hPSCs) have the ability to advance specificity of in vitro assays for drug discovery and safety pharmacology. They may also provide a superior cell source for ...
-
Article
Open AccessBioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections
The increasing number of severe infections with multi-drug-resistant pathogens worldwide highlights the need for alternative treatment options. Given the pivotal role of phagocytes and especially alveolar macr...
-
Article
Open AccessLarge-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
Megakaryocytes (MKs) are the precursors of platelets (PLTs) and may be used for PLT production in vivo or in vitro, as well as a source for PLT-derived growth factors. Induced pluripotent stem cells represent an ...
-
Article
Open AccessExpansion of functional personalized cells with specific transgene combinations
Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses ...
-
Book
