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Open AccessEfficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
Porcine reproductive and respiratory syndrome virus (PRRSV) infection can cause severe reproductive failure in sows and respiratory distress in pigs of all ages, leading to major economic losses. To date, ther...
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Article
Open AccessDevelopment of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses
H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have clos...
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Article
Open AccessGeneration and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system
Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and Erns are ma...
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Article
Open AccessDevelopment and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever viruses
African swine fever (ASF) is the most important disease to the pigs and cause serious economic losses to the countries with large-scale swine production. Vaccines are recognized as the most useful tool to prev...
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Article
Open AccessPCV cap proteins fused with calreticulin expressed into polymers in Escherichia coli with high immunogenicity in mice
Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus diseases (PCVDs) which causes huge yearly economic losses in the swine industry. Capsid protein (Cap) is the major structural ...
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Article
A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres
A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The Au...
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Article
Open AccessPorcine sialoadhesin suppresses type I interferon production to support porcine reproductive and respiratory syndrome virus infection
Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant threat to the global swine industry. Porcine sialoadhesin (poSn) has been previously shown to mediate PRRSV attachment and internali...
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Article
Open AccessPhylogenetic analysis of porcine circovirus type 2 (PCV2) between 2015 and 2018 in Henan Province, China
Porcine circovirus type 2 (PCV2) is the pathogen of porcine circovirus associated diseases (PCVAD) and one of the main pathogens in the global pig industry, which has brought huge economic losses to the pig in...
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Article
Open AccessDetection and genetic characteristics of porcine circovirus 3 based on oral fluids from asymptomatic pigs in central China
Porcine circovirus 3 (PCV3) is an emerging etiological agent to the swine industry. However, its circulating status and genetic characteristics were still unclear in Henan, central China. Here, 318 porcine ora...
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Article
Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China
Porcine circovirus type 2 (PCV2) is an economically important pathogen in domestic pigs and wild boars all around the world. To understand the molecular epidemiology of PCV2 strains circulating in central Chin...
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Article
A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for...
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Article
Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen β-Conglycinin
A rapid lateral flow colloidal gold immunoassay strip was established in a sandwich format with the colloidal gold-labelled mouse anti-β-conglycinin monoclonal antibody (mAb) and the rabbit anti-β-conglycinin ...
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Article
Open AccessDetection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene
Porcine epidemic diarrhea (PED) has increased in severity in China since 2010. To investigate further the infectivity, genetic diversity and molecular epidemiology of its causative agent, the porcine epidemic ...
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Article
Open AccessNonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that...
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Article
High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli
To improve the expression of soluble IBDV VP2 protein by using different tagged vectors in Escherichia coli.
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Article
Identification of host cell binding peptide from an overlap** peptide library for inhibition of classical swine fever virus infection
The envelope proteins of classical swine fever virus (CSFV) mediate the binding of CSFV to cell surface molecules and allow CSFV subsequent to enter host cells. However, the proteins binding to host cells and ...
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Article
Erratum to: Marek’s disease virus-encoded microRNAs: genomics, expression and function
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Article
Marek’s disease virus-encoded microRNAs: genomics, expression and function
MicroRNAs (miRNAs) are the recently discovered small non-coding RNA molecules that have post-transcriptional regulatory functions in many important biological processes. A large number of miRNAs have been foun...
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Article
Characterization and ligand specificity of sheep IgG2 receptor
Neutrophils and macrophages in cattle express a novel class of immunoglobulin Fc receptor, specific for bovine IgG2, termed boFcγ2R. In cows, the ability of neutrophils to kill immunoglobulin-opsonized microor...