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Article
Open AccessInsights into the mechanism of SARS-CoV-2 main protease autocatalytic maturation from model precursors
A critical step for SARS-CoV-2 assembly and maturation involves the autoactivation of the main protease (MProWT) from precursor polyproteins. Upon expression, a model precursor of MProWT mediates its own release ...
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Article
Open AccessAuthor Correction: Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells
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Article
Open AccessArchitectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells
Proper organization of intracellular assemblies is fundamental for efficient promotion of biochemical processes and optimal assembly functionality. Although advances in imaging technologies have shed light on ...
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Article
Open AccessAutoprocessing and oxyanion loop reorganization upon GC373 and nirmatrelvir binding of monomeric SARS-CoV-2 main protease catalytic domain
The monomeric catalytic domain (residues 1–199) of SARS-CoV-2 main protease (MPro1-199) fused to 25 amino acids of its flanking nsp4 region mediates its autoprocessing at the nsp4-MPro1-199 junction. We report th...
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Article
Open AccessModulation of the monomer-dimer equilibrium and catalytic activity of SARS-CoV-2 main protease by a transition-state analog inhibitor
The role of dimer formation for the onset of catalytic activity of SARS-CoV-2 main protease (MProWT) was assessed using a predominantly monomeric mutant (MProM). Rates of MProWT and MProM catalyzed hydrolyses dis...
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Article
Open AccessMolecular architecture of a cylindrical self-assembly at human centrosomes
The cell is constructed by higher-order structures and organelles through complex interactions among distinct structural constituents. The centrosome is a membraneless organelle composed of two microtubule-der...
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Article
Open AccessSirt1 carboxyl-domain is an ATP-repressible domain that is transferrable to other proteins
Sirt1 is an NAD+-dependent protein deacetylase that regulates many physiological functions, including stress resistance, adipogenesis, cell senescence and energy production. Sirt1 can be activated by energy depri...
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Article
Open Access‘AND’ logic gates at work: Crystal structure of Rad53 bound to Dbf4 and Cdc7
Forkhead-associated (FHA) domains are phosphopeptide recognition modules found in many signaling proteins. The Saccharomyces cerevisiae protein kinase Rad53 is a key regulator of the DNA damage checkpoint and use...
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Article
Open AccessFarnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions
Prenylated proteins play key roles in several human diseases including cancer, atherosclerosis and Alzheimer’s disease. KRAS4b, which is frequently mutated in pancreatic, colon and lung cancers, is processed b...
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Article
Complete dissociation of the HIV-1 gp41 ectodomain and membrane proximal regions upon phospholipid binding
The envelope glycoprotein gp41 mediates the process of membrane fusion that enables entry of the HIV-1 virus into the host cell. Strong lipid affinity of the ectodomain suggests that its heptad repeat regions ...
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Article
Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds
Multivalent effects dictate the binding affinity of multiple ligands on one molecular entity to receptors. Integrins are receptors that mediate cell attachment through multivalent binding to peptide sequences ...
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Article
Atomic-resolution dynamics on the surface of amyloid-β protofibrils probed by solution NMR
When proteins associate with larger structures such as polymers, membranes or solid supports, they usually become 'invisible' to the techniques used to visualize them as free molecules in solution. Marius Clor...
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Article
Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3
Centromeres, regions on the chromosome that are essential for accurate chromosome segregation, contain unique chromatin that is marked by a histone H3 variant termed CenH3 or CENP-A. The simple centromeres of ...
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Article
Maintenance of a constitutive heterochromatin domain in vertebrates by a Dicer-dependent mechanism
Small RNAs are known to induce heterochromatin formation in various organisms. RNA-dependent mechanisms are shown to be required for the formation of a constitutive heterochromatin structure in the chick.
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Article
The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla
The Hedgehog signalling pathway specifies tissue pattern in metazoan embryos. Intriguingly, the crystal structure of the human Sonic Hedgehog protein Shh complexed with a fragment of the cell surface protein C...
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Article
Structural basis for DNA bridging by barrier-to-autointegration factor
The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better u...
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Article
Molecular architecture of a eukaryotic DNA transposase
Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of str...
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Article
Insights into negative modulation of E. coli replication initiation from the structure of SeqA–hemimethylated DNA complex
The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-...
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The molecular specificity of IgG-Fc interactions with Fcγ receptors
The molecular specificity and mechanisms for the interaction of immunoglobulins with Fc binding ligands has been a subject of intensive investigation for many years. Significant information has been gained for...
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Article
Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration
The solution structure of the human barrier-to-autointegration factor, BAF, a 21,000 Mr dimer, has been solved by NMR, including extensive use of dipolar couplings which provide a priori long range structural inf...
