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Article
Open AccessRevealing protonation states and tracking substrate in serine hydroxymethyltransferase with room-temperature X-ray and neutron crystallography
Pyridoxal 5’-phosphate (PLP)-dependent enzymes utilize a vitamin B6-derived cofactor to perform a myriad of chemical transformations on amino acids and other small molecules. Some PLP-dependent enzymes, such as s...
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Article
Open AccessSeverity of COVID-19 in children with cancer: Report from the United Kingdom Paediatric Coronavirus Cancer Monitoring Project
Children with cancer are frequently immunocompromised. While children are generally thought to be at less risk of severe SARS-CoV-2 infection than adults, comprehensive population-based evidence for the risk i...
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Article
Open AccessOxygen reactivity with pyridoxal 5′-phosphate enzymes: biochemical implications and functional relevance
The versatility of reactions catalyzed by pyridoxal 5′-phosphate (PLP) enzymes is largely due to the chemistry of their extraordinary catalyst. PLP is necessary for many reactions involving amino acids. Reacti...
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Article
Open AccessDoes routine surveillance imaging after completing treatment for childhood solid tumours cause more harm than good? A systematic review and meta-analysis protocol
This systemic review aims to synthesise the current literature surrounding off-therapy surveillance imaging in children and young people with extra-cranial solid tumours, with a view to establishing if routine...
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Article
The entropic force generated by intrinsically disordered segments tunes protein function
Protein structures are dynamic and can explore a large conformational landscape1,2. Only some of these structural substates are important for protein function (such as ligand binding, catalysis and regulation)3–5
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Open AccessMeta-ethnography of experiences of early discharge, with a focus on paediatric febrile neutropenia
Many children have no significant sequelae of febrile neutropenia. A systematic review of clinical studies demonstrated patients at low risk of septic complications can be safely treated as outpatients using o...
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Article
Phosphorylation of pyridoxal 5′-phosphate enzymes: an intriguing and neglected topic
Pyridoxal 5′-phosphate (PLP)-dependent enzymes catalyze a wide range of reactions of amino acids and amines, with the exception of glycogen phosphorylase which exhibits peculiar both substrate preference and c...
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Article
Bowing fracture of the inferior angle of the scapula, a difficult diagnosis
A 4-year-old boy presented with swelling over the inferior tip of the scapula and an unclear history. Initial radiographic findings were concerning for an aggressive lesion. This case highlights how a multimod...
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Article
Open AccessSurvival rates and prognostic predictors of high grade brain stem gliomas in childhood: a systematic review and meta-analysis
Diagnosis of a pediatric high grade brain stem glioma is devastating with dismal outcomes. This systematic review and meta-analysis was undertaken to determine the survival rates and assess potential prognosti...
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Article
Critical review of current clinical practice guidelines for antifungal therapy in paediatric haematology and oncology
The incidence of invasive fungal disease (IFD) is rising, but its treatment in paediatric haematology and oncology patients is not yet standardised. This review aimed to critically appraise and analyse the cli...
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Article
Inhibition of tyrosine phenol-lyase by tyrosine homologues
We have designed, synthesized, and evaluated tyrosine homologues and their O-methyl derivatives as potential inhibitors for tyrosine phenol lyase (TPL, E.C. 4.1.99.2). Recently, we reported that homologues of try...
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Article
Erratum: Predicting microbiologically defined infection in febrile neutropenic episodes in children: global individual participant data multivariable meta-analysis
Correction to: British Journal of Cancer (2016) 114, 623–630. doi:10.1038/bjc.2016.28; published online 8 March 2016 Upon publication of the above paper in the British Journal of Cancer, the authors identified...
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Article
Systematic review of reduced therapy regimens for children with low risk febrile neutropenia
Reduced intensity therapy for children with low-risk febrile neutropenia may provide benefits to both patients and the health service. We have explored the safety of these regimens and the effect of timing of ...
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Article
Open AccessPredicting microbiologically defined infection in febrile neutropenic episodes in children: global individual participant data multivariable meta-analysis
Risk-stratified management of fever with neutropenia (FN), allows intensive management of high-risk cases and early discharge of low-risk cases. No single, internationally validated, prediction model of the ri...
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Article
Open AccessAminoaciduria in the prediction of ifosfamide-induced tubulopathy after childhood cancer: a feasibility study
Ifosfamide, an alkylating agent used widely in the treatment of childhood malignancy, can cause many side effects including a proximal tubulopathy. Studies suggest that aminoaciduria is seen most commonly of a...
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Article
Open AccessProtocol for a systematic review of reductions in therapy for children with low-risk febrile neutropenia
Febrile neutropenia is a common complication of therapy in children with cancer. Some patients are at low risk of complications, and research has considered reduction in therapy for these patients. A previous ...
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Article
Preparation of 3-bromo-l-tyrosine and 3,5-dibromo-l-tyrosine
l-Tyrosine is converted to 3-bromo-l-tyrosine in good yield by reaction with 1.2 equiv. of DMSO in HBr/AcOH, while reaction with 2.2 equiv. of DMSO under comparable conditions results in formatio...
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Article
Open AccessPredicting infectious complications in neutropenic children and young people with cancer (IPD protocol)
A common and potentially life-threatening complication of the treatment of childhood cancer is infection, which frequently presents as fever with neutropenia. The standard management of such episodes is the ex...
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Article
Open AccessSystematic review and meta-analysis of the value of initial biomarkers in predicting adverse outcome in febrile neutropenic episodes in children and young people with cancer
Febrile neutropenia is a frequently occurring and occasionally life-threatening complication of treatment for childhood cancer. Many biomarkers have been proposed as predictors of adverse events. We aimed to u...
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Article
Open AccessConformational changes and loose packing promote E. coli Tryptophanase cold lability
Oligomeric enzymes can undergo a reversible loss of activity at low temperatures. One such enzyme is tryptophanase (Trpase) from Escherichia coli. Trpase is a pyridoxal phosphate (PLP)-dependent tetrameric enzyme...
