22 Result(s)
within
Robert G Parton
Science, Humanities and Social Sciences, multidisciplinary
-
Article
Open AccessApicobasal RNA asymmetries regulate cell fate in the early mouse embryo
The spatial sorting of RNA transcripts is fundamental for the refinement of gene expression to distinct subcellular regions. Although, in non-mammalian early embryogenesis, differential RNA localisation presag...
-
Article
Open AccessIntegrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria
Even in the setting of optimal resuscitation in high-income countries severe sepsis and septic shock have a mortality of 20–40%, with antibiotic resistance dramatically increasing this mortality risk. To devel...
-
Article
Open AccessSugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts
Osteoclasts are giant bone-digesting cells that harbor specialized lysosome-related organelles termed secretory lysosomes (SLs). SLs store cathepsin K and serve as a membrane precursor to the ruffled border, t...
-
Article
Open AccessPublisher Correction: Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis
-
Article
Open AccessNicotinamide riboside attenuates age-associated metabolic and functional changes in hematopoietic stem cells
With age, hematopoietic stem cells (HSC) undergo changes in function, including reduced regenerative potential and loss of quiescence, which is accompanied by a significant expansion of the stem cell pool that...
-
Article
Open AccessHigh intraluminal pressure promotes vascular inflammation via caveolin-1
The aetiology and progression of hypertension involves various endogenous systems, such as the renin angiotensin system, the sympathetic nervous system, and endothelial dysfunction. Recent data suggest that va...
-
Article
Open AccessCavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
Caveolae are spherically shaped nanodomains of the plasma membrane, generated by cooperative assembly of caveolin and cavin proteins. Cavins are cytosolic peripheral membrane proteins with negatively charged i...
-
Article
Open AccessIn vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation
The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formatio...
-
Article
Open AccessReactivation of Myc transcription in the mouse heart unlocks its proliferative capacity
It is unclear why some tissues are refractory to the mitogenic effects of the oncogene Myc. Here we show that Myc activation induces rapid transcriptional responses followed by proliferation in some, but not a...
-
Article
Open AccessAn Abl-FBP17 mechanosensing system couples local plasma membrane curvature and stress fiber remodeling during mechanoadaptation
Cells remodel their structure in response to mechanical strain. However, how mechanical forces are translated into biochemical signals that coordinate the structural changes observed at the plasma membrane (PM...
-
Article
Open AccessIdentification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis
Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae di...
-
Article
Open AccessMechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here...
-
Article
Open AccessSmall GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis
Using real-time TIRF microscopy imaging, we identify sites of clathrin and dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows spatio-temporal localisation of known molecules affecting ...
-
Article
Open AccessAuthor Correction: Tyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1
A correction to this article has been published and is linked from the HTML version of this article.
-
Article
Open AccessTyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1
Contractile adherens junctions support cell−cell adhesion, epithelial integrity, and morphogenesis. Much effort has been devoted to understanding how contractility is established; however, less is known about ...
-
Article
Open AccessORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P 2) and regulate its level at the plasma membrane
ORP5 and ORP8, members of the oxysterol-binding protein (OSBP)-related proteins (ORP) family, are endoplasmic reticulum membrane proteins implicated in lipid trafficking. ORP5 and ORP8 are reported to localize...
-
Article
Open AccessLaser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis
Remarkably little is known about how intracellular pathogens exit the host cell in order to infect new hosts. Pathogenic chlamydiae egress by first rupturing their replicative niche (the inclusion) before rapi...
-
Article
Open AccessNanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence
Protein aggregation is a hallmark of many neurodegenerative diseases, notably Alzheimer’s and Parkinson’s disease. Parkinson’s disease is characterized by the presence of Lewy bodies, abnormal aggregates mainl...
-
Article
Erratum: Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Nature 526, 564–568 (2015); doi:10.1038/nature15695 In the Methods of this Letter, ‘5,000’ should have read ‘15,000’ in the sentence: “Then, cells were again plated on a Matrigel-coated at 15,000 cells per cm2...
-
Article
Open AccessInterplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We repor...
