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Article
Open AccessApicobasal RNA asymmetries regulate cell fate in the early mouse embryo
The spatial sorting of RNA transcripts is fundamental for the refinement of gene expression to distinct subcellular regions. Although, in non-mammalian early embryogenesis, differential RNA localisation presag...
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Article
Open AccessIn vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation
The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formatio...
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Article
Open AccessIdentification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis
Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae di...
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Article
Open AccessORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P 2) and regulate its level at the plasma membrane
ORP5 and ORP8, members of the oxysterol-binding protein (OSBP)-related proteins (ORP) family, are endoplasmic reticulum membrane proteins implicated in lipid trafficking. ORP5 and ORP8 are reported to localize...
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Article
Cavin-1 deficiency modifies myocardial and coronary function, stretch responses and ischaemic tolerance: roles of NOS over-activity
Caveolae and associated cavin and caveolins may govern myocardial function, together with responses to mechanical and ischaemic stresses. Abnormalities in these proteins are also implicated in different cardio...
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Article
Open AccessLaser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis
Remarkably little is known about how intracellular pathogens exit the host cell in order to infect new hosts. Pathogenic chlamydiae egress by first rupturing their replicative niche (the inclusion) before rapi...
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Article
An endosomal tether undergoes an entropic collapse to bring vesicles together
A new endosomal tethering mechanism involving a mechanochemical cycle of the dimeric coiled-coil protein EEA1 regulated by Rab5:GTP binding and GTP hydrolysis.
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Article
Erratum: Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Nature 526, 564–568 (2015); doi:10.1038/nature15695 In the Methods of this Letter, ‘5,000’ should have read ‘15,000’ in the sentence: “Then, cells were again plated on a Matrigel-coated at 15,000 cells per cm2...
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Article
Open AccessInterplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We repor...
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Article
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
The kidney arises from two types of progenitors; here, the signalling conditions that induce the production of collecting ducts and functional nephrons from human pluripotent stem cells are determined, and org...
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Article
Open AccessAMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation
Lipid droplets (LDs) are intracellular organelles that provide fatty acids (FAs) to cellular processes including synthesis of membranes and production of metabolic energy. While known to move bidirectionally a...
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Article
Do not phase out nuclear power — yet
Fission power must remain a crucial part of the energy mix until renewable energy technologies can be scaled up, argues Charles D. Ferguson.
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Article
Myosin II isoforms identify distinct functional modules that support integrity of the epithelial zonula adherens
Different myosin II isoforms have distinct roles at adherens junctions: myosin IIa and IIb localization to junctions is regulated by unique upstream signals and they control specific aspects of junction adhesi...
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Article
Endosome-to-cytosol transport of viral nucleocapsids
During viral infection, fusion of the viral envelope with endosomal membranes and nucleocapsid release were thought to be concomitant events. We show here that for the vesicular stomatitis virus they occur seq...
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Article
A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans
Galactomannan was coupled to a protein carrier for the preparation of monoclonal antibodies. The monoclonal antibodies generated bound to galactomannans from different sources as well as to glucomannan and ga...
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Links in the Chain: The Contribution of Kettin to the Elasticity of Insect Muscles
Asynchronous flight muscle fibers are activated by periodic stretches and need to be stiff for strain to be transmitted to the contractile system. Kettin associated with thin filaments and projectin with thick...
