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Reference Work Entry In depth
Streptokinase Production in Yeast Systems
Streptokinase is a fibrinolytic agent widely used in thrombosis. The clinical trials and experimental studies proved that the SK is a safe and inexpensive thrombolytic medicine compared with its homologues suc...
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Living Reference Work Entry In depth
Streptokinase Production in Yeast Systems
Streptokinase is a fibrinolytic agent widely used in thrombosis. The clinical trials and experimental studies proved that the SK is a safe and inexpensive thrombolytic medicine compared with its homologues suc...
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Article
Open AccessPurification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.
Two alpha-galactosidases (Ag-I & Ag-II) were purified from Acinetobacter sp. Both the enzymes were monomeric with pH optima of 7.0 and molecular weight of 65 kDa for Ag-I and 37 kDa for Ag-II. The temperature op...
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Article
Reducing Sugars Production from Corncobs: A Comparative Study of Chemical and Biotechnological Methods
Two commonly used chemical pretreatment processes, sulphuric acid, and sodium hydroxide, were tested to provide comparative performance data. A connection between solid to liquid ratio (S/L) and sugars release...
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Article
Chemometrics-Based Process Analytical Technology (PAT) Tools: Applications and Adaptation in Pharmaceutical and Biopharmaceutical Industries
Process analytical technology (PAT) is used to monitor and control critical process parameters in raw materials and in-process products to maintain the critical quality attributes and build quality into the pr...
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Chapter
Fermentable Sugars from Lignocellulosic Biomass: Technical Challenges
Lignocelluloses, the most abundant renewable biomass on earth, are composed mainly of cellulose, hemicellulose, and lignin. Both the cellulose and hemicellulose fractions are polymers of sugars and thereby a p...
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Article
Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™
Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillu...
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Article
Continuous Production of Oxytetracycline in Fluidized Bed Bioreactor by Immobilized Cells of Streptomyces varsoviensis MTCC-1537: Effect of Dilution and Glucose Loading Rates
Oxytetracycline (OT) production using glutaraldehyde cross-linked calcium alginate immobilized cells of Streptomyces varsoviensis in continuous fluidized bed reactor (FBR) was investigated. Initially, batch exper...
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Article
Multiple Responses Optimization and Modeling of Lipase Production by Rhodotorula mucilaginosa MTCC-8737 Using Response Surface Methodology
Response surface methodology was employed to optimize culture medium for production of lipase with Rhodotorula sp. MTCC 8737. In the first step, a Plackett–Burman design was used to evaluate the effects of differ...
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Article
Constitutive Expression and Optimization of Nutrients for Streptokinase Production by Pichia pastoris Using Statistical Methods
The Pichia pastoris clone producing streptokinase (SK) was optimized for its nutritional requirements to improve intracellular expression using statistical experimental designs and response surface methodology. T...
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Article
Effect of Aeration and Agitation Regimes on Lipase Production by Newly Isolated Rhodotorula mucilaginosa–MTCC 8737 in Stirred Tank Reactor Using Molasses as Sole Production Medium
The influence of media and process parameters (aeration and agitation) on fermentation broth rheology and biomass formation has been studied in 1.5-l stirred tank reactor for lipase production using Rhodotorula m...
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Article
Biological Treatment of Toxic Petroleum Spent Caustic in Fluidized Bed Bioreactor Using Immobilized Cells of Thiobacillus RAI01
In the present studies, newly isolated Thiobacillus sp was used for the treatment of synthetic spent sulfide caustic in a laboratory-scale fluidized bed bioreactor. The sulfide oxidation was tested using Ca-algin...