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Article
Evaluation of DNA damage by alkaline elution technique after inhalation exposure of rats and mice to 1,3-butadiene
The alkaline filter elution technique was used to evaluate single strand breaks (SSB), DNA-DNA (DDCL) and DNA-protein cross-links (DPCL) in liver and lung of male rats (Sprague-Dawley) and male mice (B6C3F1) a...
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Article
Pharmacokinetic interaction between 1,3-butadiene and styrene in Sprague-Dawley rats
Gas uptake studies were carried out to evaluate kinetic interactions between 1,3-butadiene and styrene in Sprague-Dawley rats. The animals were co-exposed by inhalation to a mixture of 1,3-butadiene between 20...
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Article
Investigation of species differences in isobutene (2-methylpropene) metabolism between mice and rats
Metabolism of isobutene (2-methylpropene) in rats (Sprague Dawley) and mice (B6C3F1) follows kinetics according to Michaelis-Menten. The maximal metabolic elimination rates are 340 μmol/kg/h for rats and 560 μmol...
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Article
Use of linear free energy relationships in toxicology: prediction of partition coefficients of volatile lipophilic compounds
A relationship between the partition coefficient (P) and the boiling point (TBp, expressed in degrees Kelvin) was derived as $$T_{Bp} ...
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Article
Detection of diepoxybutane-induced DNA-DNA crosslinks by cesium trifluoracetate (CsTFA) density-gradient centrifugation
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Article
32P-postlabelling method as a sensitive indicator for analysis of genotoxicity of biphenyl derivatives
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Chapter and Conference Paper
Species Differences in DNA Damage by Butadiene: Role of Diepoxybutane
In microsomal incubates 1,3-butadiene (BD) is metabolized by cytochrome P- 450 to its epoxide 1,2-epoxybutene (EB). Further metabolic transformation of the epoxide intermediate by epoxide hydrolase and/or mono...
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Chapter and Conference Paper
Species Differences in Pharmacokinetics, Metabolism, and DNA Binding of Inhaled 1,3-Butadiene
1,3-Butadiene, a major component in synthetic rubber and adiponitrile manufacture, has been found to produce cancer at multiple anatomic sites in two species of experimental animals. In an inhalation bioassay ...
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Article
Inhalation pharmacokinetics of 1,2-epoxybutene-3 reveal species differences between rats and mice sensitive to butadiene-induced carcinogenesis
Comparative investigations of inhalation pharmacokinetics of 1,2-epoxybutene-3 (vinyl oxirane, the primary reactive intermediate of butadiene) revealed major differences in metabolism of this compound between ...
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Article
DNA adducts of halogenated hydrocarbons
Although formation of DNA adducts has been postulated for several halomethanes, no chemical identification of such adducts has been performed so far. There is, however, evidence that methyl chloride does not a...
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Article
Species differences in butadiene metabolism between mice and rats evaluated by inhalation pharmacokinetics
Metabolism of 1,3-butadiene to 1,2-epoxybutene-3 in rats follows saturation kinetics. Comparative investigation of inhalation pharmacokinetics in mice also revealed a saturation pattern. For both species “line...
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Chapter and Conference Paper
Vinyl Acetate, a Structural Analog of Vinyl Carbamate, Fails to Induce Enzyme Altered Foci in Rat Liver
Contradictory reports on a possible carcinogenicity of vinyl acetate (VAC) have caused concern about the metabolism and toxicity of this compound. Whereas the findings with VAC were negative in an inhalation b...
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Article
DNA-binding assay of methyl chloride
Fischer-344 rats and B6C3F1 mice of both sexes were exposed in closed chambers to 14C-labeled methyl chloride. Different clearance values from the gas phase of the system indicated that, based on body weight, mic...
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Chapter
Covalent Binding of Haloethylenes
Halogenated ethylenes are metabolized to reactive intermediates which covalently bind to different cellular targets.
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Chapter and Conference Paper
Alkylation of RNA by Vinyl Chloride and Vinyl Bromide Metabolites in Vivo: Effect on Protein Biosynthesis
Alkylation of DNA is viewed as representing the initial critical step in carcinogenesis induced by chemical substances. Vinyl chloride and vinyl bromide, compounds with proven carcinogenic potency toward the l...
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Chapter and Conference Paper
Metabolic Activation and Pharmacokinetics in Hazard Assessment of Halogenated Ethylenes
Halogenated ethylenes play an important role in occupational and environmental medicine. Several attempts at toxicological evaluation of such compounds have been recently published (Maltoni 1977; Lee et al. 19...
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Article
Alkylation of RNA by vinyl bromide metabolites in vitro and in vivo
[1,2-14C]Vinyl bromide was incubated with rat liver microsomes, NADPH, and polyadenylic acid, polycytidylic acid, or RNA, respectively. Part of the adenosine moieties in RNA or in polyadenylic acid were alkylated...
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Article
Vinyl chloride and trichloroethylene: Comparison of alkylating effects of metabolites and induction of preneoplastic enzyme deficiencies in rat liver
[1,2-14C] Vinyl chloride and [1,2-14C] trichloroethylene were incubated with rat liver microsomes, NADPH and RNA (from yeast). Whereas trichloroethylene metabolites were irreversibly bound to proteins in microsom...
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Chapter and Conference Paper
Induction of Pre-Malignant Hepatic Lesions: Comparative Effects of Vinyl Chloride and Trichloroethylene
Formation of hemangioendotheliomas of liver in man and in experimental animals after exposure to vinyl chloride has focussed attention on possible cancerogenic effects of halogenated ethylenes. Vinyl chloride ...
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Article
Formation of 3,N4-ethenocytidine moieties in RNA by vinyl chloride metabolites in vitro and in vivo
Rats were exposed to [1,2-14C] vinyl chloride. Liver RNA was isolated, hydrolyzed, and the nucleosides separated on Aminex-A-6. Besides the physiological bases and 1,N6-ethenoadenosine, radioactivity was also inc...
