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Article
Genetic and epigenetic integrity assessment of acclimatised papaya plants regenerated directly from shoot-tips following short- and long-term cryopreservation
A vitrification-based cryopreservation protocol was applied to in vitro sourced shoot-tips of four genotypes of Carica papaya; two female (70 and Z6) and two male (B2 and B4). Regeneration of ~58 % (70) and ~59 %...
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Article
Papaya ringspot virus resistance in Carica papaya via introgression from Vasconcellea quercifolia
We report the first successful production of PRSV-P resistant backcross (BC) papaya plants following intergeneric hybridisation between C. papaya and a Vasconcellea species after 50 years of reports on unsuccessf...
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Article
Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones
This paper is the first report of field performance and evaluation of morphological traits following cryopreservation in four genotypes of Carica papaya (Z6, 97, TS2 and 35). It also describes the successful esta...
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Article
Assessment of genetic and epigenetic changes following cryopreservation in papaya
A vitrification based cryopreservation technique for storage of in vitro shoot tips of papaya has been tested to ensure applicability across a range of genotypes and to assess the stability of both genotype an...
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Article
Development of a codominant CAPS marker linked to PRSV-P resistance in highland papaya
Development of resistant papaya varieties is widely considered the best strategy for long-term control of the papaya ringspot virus type P (PRSV-P). Several species of “highland papaya” from the related genus Vas...
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Article
Maternal inheritance of cytoplasmic organelles in intergeneric hybrids of Carica papaya L. and Vasconcellea spp. (Caricaceae Dumort., Brassicales)
The mode of inheritance of chloroplast and mitochondrial DNA has been determined in intergeneric hybrids between C. papaya and four different Vasconcellea species by employing a restriction fragment length polymo...
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Article
RAPD and isozyme analysis of genetic relationships between Carica papaya and wild relatives
The genetic origin of cultivated papaya is not clear. Wild relatives of papaya (Carica papaya) from central southern America were investigated using isozyme and RAPD analysis. Seven other species (including si...
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Article
Morphological, molecular and cytological analyses of Carica papaya×C. cauliflora interspecific hybrids
Morphological, molecular and cytological analyses were performed to assess the hybridity of 120 putative interspecific hybrids of Carica papaya L.×C. cauliflora Jacq. In the putative interspecific hybrids the nu...
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Chapter
Micropropagation of Passiflora Species (Passionfruit)
The genus Passiflora (family Passifloraceae) comprises 400 species, of which 50 to 60 bear edible fruits (Martin and Nakasone 1970); however, the genus is as well known for its colourful flowers. Most of the Pass...
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Chapter
Application of Biotechnology to Carica Papaya and Related Species
An efficient protocol has been developed for clonal multiplication of papaya based on rooted microcuttings from nodes of apically dominant plants in vitro. Other Carica species cauliflora, parviflora, pubescens a...
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Article
Field evaluation of micropropagated bananas derived from plants containing Banana Bunchy-Top Virus
Micropropagated bananas derived from Banana Bunchy-Top Virus (BBTV) infected plants, but displaying no symptoms of the disease, were established in the field. They were grown for three years and produced a pla...
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Article
Effect of explant type on proliferation of Carica papaya L. in vitro
Three papaya explant types (entire plantlets, plantlets with apices removed, or roots removed) were cultured on media with and without 2 μM BAP and 0.5 μM NAA. Best growth of lateral shoots occurred in the pre...
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Article
The transmission of banana bunchy-top virus in micropropagated bananas
Plants were established in vitro from banana bunchy0top virus (BBTV) infeeted plants. Explants containing either vegetative shoot apices or terminal floral apices were used to initiate cultures. Plants multipl...