Abstract
Three papaya explant types (entire plantlets, plantlets with apices removed, or roots removed) were cultured on media with and without 2 μM BAP and 0.5 μM NAA. Best growth of lateral shoots occurred in the presence of exogenous hormones, on plants with apex removed but roots intact. The presence of roots increased the number of lateral shoots formed, irrespective of the presence or absence of the apex. Root initiation on harvested lateral shoots was significantly reduced (P<0.01) when shoot length was<5 mm. Rooted shoots grew rapidly into apically dominant plantlets before subculture to multiplication medium. This procedure has produced a technique that could produce 10 000 microcuttings in vitro from one plantlet in a year, and should be free of genetic off-types.
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Miller, R.M., Drew, R.A. Effect of explant type on proliferation of Carica papaya L. in vitro. Plant Cell Tiss Organ Cult 21, 39–44 (1990). https://doi.org/10.1007/BF00034489
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DOI: https://doi.org/10.1007/BF00034489