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Article
The Arabidopsis nuclear DAL gene encodes a chloroplast protein which is required for the maturation of the plastid ribosomal RNAs and is essential for chloroplast differentiation
Altered pigmentation is an easily scored and sensitive monitor of plastid function. We analyzed in detail a yellow colored transposon-tagged mutant (dal1-2) that is allelic to the dal mutant previously identified...
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Article
Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein
In order to study the regulation of nuclear genes coding for plastid ribosomal proteins, we have analysed the promoter region of spinach rps22 using both in vitro and in vivo approaches. By footprinting analyses,...
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Chapter
The Spinach Plastid Ribosome: Protein Properties and Aspects of Ribosome Biosynthesis
Plastid ribosomes consist of components which have generally kept a high degree of homology with their prokaryotic ancestors. All types of rRNAs (Kössel, 1991) and most of the ribosomal proteins (for review se...
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Article
Organization and expression of the nuclear gene coding for the plastid-specific S22 ribosomal protein from spinach
We report here on the genomic organization and expression of a nuclear gene coding for a plastid ribosomal protein. The gene encodes the plastid-specific ribosomal protein S22 (formerly named CS-S5). Southern ...
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Article
Characterization of pollen polygalacturonase encoded by several cDNA clones in maize
A full-length cDNA clone, named PG1, abundantly expressed in late stages of pollen development, has been isolated from a cDNA library using a differential screening method with cDNA probes representative of mi...
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Article
Nucleotide sequence and expression of a novel glycine-rich protein gene from Arabidopsis thaliana
A clone containing a gene coding for a novel glycine-rich protein has been identified in an Arabidopsis thaliana genomic library. The gene codes for a 339 amino acid protein and is interrupted by a 686 bp interve...
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Article
Presence in the stroma of chloroplasts of a large pool of a ribosomal protein not structurally related to any Escherichia coli ribosomal protein
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Article
Evidence for a composite phylogenetic origin of the plastid genome of the brown alga Pylaiella littoralis (L.) Kjellm
The nucleotide sequence and the 5′ flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and t...
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Article
Presence in the stroma of chloroplasts of a large pool of a ribosomal protein not structurally related to any Escherichia coli ribosomal protein
A search was made for the presence of a pool of free ribosomal proteins in the stroma of the spinach chloroplast. The results showed that a relatively large amount of one protein, CS-S5, is present in the stro...
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Article
Cotranscription of the S10- and spc-like operons in spinach chloroplasts and identification of three of their gene products
The organisation and expression of the rpl22, rps3, rpl16 and rpl14 genes, which belong to the S10- and spc-like operons of spinach chloroplasts, have been studied. Northern experiments and nuclease S1 map** sh...
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Article
Plastid genes inPylaiella littoralis
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Article
The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants
The expression of components of the 70S plastid ribosome has been determined during the first 13 days of spinach plant development. Total cellular RNA and proteins were used to determine the relative steady-st...
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Article
Identification of an Escherichia coli S1-like protein in the spinach chloroplast ribosome
Antibodies directed against E. coli ribosomal protein S1 were used in immunoblotting assays to search for an S1-like protein in the ribosome of spinach chloroplast. An immunological cross-reaction was reproducibl...
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Article
Transcription study of the genes encoded in the region of the junction between the large single copy and the inverted repeat A of spinach chloroplast DNA
The expression of the psbA, trnH-GUG and rps19′ genes from spinach chloroplasts, coding respectively for the 32 kDa protein, the tRNAHis (GUG), and the putative ribosomal protein CS19′, has been studied by clonin...
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Article
Sequence of the plastid rDNA spacer region of the brown alga Pylaiella littoralis (L.) Kjellm. Evolutionary significance
The DNA segment situated between the 16S and 23S rRNA genes belonging to the plastid genome of the brown alga Pylaiella littoralis (L.) Kjellm. has been sequenced. This small region (322 bp) contains two unsplit ...
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Article
Structure and transcription of the 5S rRNA gene from spinach chloroplasts
The nucleotide sequence of the spinach chloroplast 5S rRNA gene and its flanking regions has been determined. A prokaryotic type promoter is to be found upstream of the 5S rRNA gene. Northern blot experiments ...
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Article
Characterization of spinach plastid ribosomal proteins by two dimensional gel electrophoresis
Ribosomes are isolated from spinach plastids using conventional sucrose gradients. Their subunits are prepared by dissociation using low Mg2+ concentration.
