-
Article
Open AccessDevelopment of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production
The production of bio-succinic acid (SA) from renewable feedstocks is a promising and sustainable approach to mitigating the high carbon emissions associated with the current energy crisis. Actinobacillus succino...
-
Article
Immobilization of Actinobacillus succinogenes on nano- and micro-fiber membranes for efficient and robust production of succinic acid
This work aimed to study the efficiency of nano- and micro- fiber membranes in immobilizing Actinobacillus succinogenes CCTCC M2012036 for succinic acid production. Among the four kinds of electrospun nanofiber m...
-
Article
Open AccessPurification and characterization of aspartic protease from Aspergillus niger and its efficient hydrolysis applications in soy protein degradation
Adding acid protease to feed can enhance protein digestibility, boost feed utilization, and stimulate the growth of animals in breading industry. In order to obtain an acid protease with high hydrolysis effici...
-
Article
Open AccessEffects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes
Succinic acid (SA), a key intermediate in the cellular tricarboxylic acid cycle (TCA), is a 4-carbon dicarboxylic acid of great industrial value. Actinobacillus succinogenes can ferment various carbon sources and...
-
Article
Simulation of Associated Particle Technique used for Reducing Noise in 14 MeV Neutron Radiography
Distortions caused by scattered neutrons and induced radiation are significant factors in fast neutron radiography (FNR). A 14 MeV fast neutron radiography system with associated particle technique (APT) has b...
-
Article
Enhanced thermostability and catalytic efficiency of glucose oxidase in Pichia Pastoris
Glucose oxidase (GOD) has many practical applications, but its poor thermostability limits its broader use. In this research, three primary mutants of wild-type GOD were designed using rational mutagenesis, an...
-
Article
Effect of the Gad system on Actinobacillus succinogenes during acid stress
The glutamate decarboxylase (Gad) system is an important amino acid-dependent acid resistance system commonly found in microorganisms. Actinobacillus succinogenes is one of the best natural producers of succinic ...
-
Article
Open AccessThe clinical value of long - term electroencephalogram (EEG) in seizure - free populations: implications from a cross-sectional study
This study aimed to explore the clinical value of long - term electroencephalogram (LTM EEG) in seizure-free individuals taking antiepileptic drugs (AEDs) for more than 2 years. We try to look for clinical fac...
-
Article
Production of a Recombinant α-l-Rhamnosidase from Aspergillus niger CCTCC M 2018240 in Pichia pastoris
α-l-Rhamnosidases have wide application in the field of biotechnology for derhamnosylation of many natural glycosides. In this study, an α-l-rhamnosidase-producing strain, Aspergillus niger CCTCC M 2018240, was i...
-
Article
Measurement of the prompt neutron spectrum from thermal-neutron-induced fission in U-235 using the recoil proton method
A measurement of the 235U prompt fission neutron spectrum (PFNS) by the recoil proton method was performed at the Institute of Nuclear Physics and Chemistry, China. Details of the method, which include the calcul...
-
Article
Construction of synthetic pathways for raspberry ketone production in engineered Escherichia coli
Raspberry ketone is an important ingredient in the flavor and fragrance industries. Due to its low content in fruits and vegetables, the production of natural raspberry ketone using heterologous synthesis in m...
-
Article
Relationship Between Secondary-Electron Yield and Structure of Polycrystalline Diamond Prepared Under Different Methane Concentrations
To understand the mechanism of electron transport and escape to vacuum of polycrystalline chemical-vapor-deposited diamond films prepared under different methane (CH4) concentrations, the secondary electron yield...
-
Article
Construction of fibrous bed bioreactor for enhanced succinic acid production using wastewater of dextran fermentation
A new applicability of wastewater of dextran fermentation (WWDF) for biological production of succinic acid with A. succinogenes CCTCC M2012036 was reported in this work for the first time. Notably, K2CO3 was use...
-
Article
Biosynthesis of Heliotropin by a Novel Strain of Serratia liquefaciens
The present study has been conducted towards isolation of bacteria capable of producing heliotropin via microbial conversion. Strain ZMT-1 capable of synthesizing heliotropin efficiently was obtained by enrich...
-
Article
Muconic acid production from glucose using enterobactin precursors in Escherichia coli
Muconic acid (MA) is a promising bulk chemical due to its extensive industrial applications in the production of adipic acid and other valuable, biodegradable intermediates. MA is heretofore mainly produced fr...
-
Article
Unfolding the fast neutron spectra of a BC501A liquid scintillation detector using GRAVEL method
Accurate knowledge of the neutron energy spectra is useful in basic research and applications. The overall procedure of measuring and unfolding the fast neutron energy spectra with BC501A liquid scintillation ...
-
Article
Enhanced succinic acid production by Actinobacillus succinogenes after genome shuffling
Succinic acid is an important platform chemical for synthesis of C4 compounds. We applied genome shuffling to improve fermentative production of succinic acid by A. succinogenes. Using a screening strategy compos...
-
Article
Spirit-based distillers’ grain as a promising raw material for succinic acid production
Spirit-based distillers’ grain (SDG) is the main by-product of the Chinese liquor industry, with an annual output of approx. 100 million tons. The economical potential of fermentative production of succinic ac...
-
Article
Genome shuffling improves thermotolerance and glutamic acid production of Corynebacteria glutamicum
Genome shuffling was used to improve the thermotolerance of l-glutamic acid-producing strain Corynebacteria glutamicum. Five strains with subtle improvements in high temperature tolerance and productivity were se...
-
Article
Display of α-amylase on the surface of Corynebacterium glutamicum cells by using NCgl1221 as the anchoring protein, and production of glutamate from starch
We developed a new cell surface display system in Corynebacterium glutamicum based on the C-terminally truncated NCgl1221 anchor protein to increase l-glutamate production from starch directly. The C-terminally t...
