Single-Cell Assays
Microfluidics, Genomics, and Drug Discovery
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Protocol
Cytosolic Calcium Measurement Utilizing a Single-Cell Biochip to Study the Effect of Curcumin and Resveratrol on a Single Glioma Cell
A microfluidic method has been developed for real-time measurement of the effects of curcumin on the intracellular calcium concentration in a single glioma cell (U87-MG). This method is based on quantitative f...
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Book
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Article
The genetic authentication of Panax ginseng and Panax quinquefolius based on using single nucleotide polymorphism (SNP) conducted in a nucleic acid test chip
Panax ginseng and Panax quinquefolius, which are commonly called Chinese ginseng and American ginseng respectively, have different medicinal properties and market values; however, these samples can be difficult ...
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Article
Quantification of Cannabinoids in Cultivars of Cannabis sp. by Gas Chromatography–Mass Spectrometry
In the chemical characterization of medically valued Cannabis, the present work has used a gas chromatography (GC) method coupled with mass spectrometry (MS) for identification and quantification of cannabinoids....
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Article
A successive laminar flow extraction for plant medicine preparation by microfluidic chip
Liquid–liquid solvent extraction is frequently used in the purification of active ingredients from plants, and repeated extraction by different solvents with different polarity is often demanded to improve pur...
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Article
NanoHDA: A nanoparticle-assisted isothermal amplification technique for genoty** assays
Isothermal methods, such as helicase-dependent amplification (HDA), have an advantage over polymerase chain reaction for DNA amplification owing to their ease of operation. Here, we developed a new HDA method ...
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Book
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Protocol
Overview of Microarray Technology
Microarray technology, with its high-throughput advantage, has been applied to analyze various biomaterials, such as nucleic acids, proteins, glycans, peptides, and cells.
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Protocol
High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device
Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the...
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Protocol
DNA Microarray-Based Diagnostics
The DNA microarray technology is currently a useful biomedical tool which has been developed for a variety of diagnostic applications. However, the development pathway has not been smooth and the technology ha...
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Article
Enhanced destabilization of mismatched DNA using gold nanoparticles offers specificity without compromising sensitivity for nucleic acid analyses
Here, we report a method that uses gold nanoparticles (AuNPs) to enhance the specificity of DNA hybridization without reducing its detection sensitivity. The conventional stringent wash method utilizes high-te...
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Reference Work Entry In depth
Microfluidic Same-Single-Cell Analysis
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Article
Same-single-cell analysis using the microfluidic biochip to reveal drug accumulation enhancement by an amphiphilic diblock copolymer drug formulation
Multidrug resistance (MDR) is one of the major obstacles in drug delivery, and it is usually responsible for unsuccessful cancer treatment. MDR may be overcome by using MDR inhibitors. Among different classes ...
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Living Reference Work Entry In depth
Microfluidic Same-Single-Cell Analysis
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Protocol
Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip
This chapter introduces a microfluidic method to study the contraction of a single cardiac muscle cell (cardiomyocyte). This method integrates single-cell selection, cell retention, dye loading, chemical stimu...
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Chapter and Conference Paper
Microfluidic Biochip Integrated with Acoustic Wave Sensor for Single Heart Muscle Cell Analysis
We report the fabrication of a microfluidic biochip integrated with an acoustic wave sensor for cell analysis. The sensor serves as a detector for measuring changes in viscoelastic properties (e.g. stiffness) ...
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Chapter and Conference Paper
Design Rule Evaluation for Micro-Scale Flow Restrictions: Comparing Experiment and Theory
Control of the direction of fluid flow within a network of flow paths in a microfluidic device is paramount to its proper operation. Pressure driven flow requires either active valving for control, or passive ...
