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    Article

    A new example of physical linkage between Tn1 and Tn21: the antibiotic multiple-resistance region of plasmid pCFF04 encoding extended-spectrum β-lactamase TEM-3

    The genetic environment of plasmid-borne bla TEM mutant genes, encoding nine distinct TEM-type extended-spectrum β-lactamases, was studied in transconjugants from clinical isolates of enterobacteria. Colony hybri...

    Claude Mabilat, Joaõ Lourençao-Vital, Sylvie Goussard in Molecular and General Genetics MGG (1992)

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    Article

    The VANA glycopeptide resistance protein is related to d-alanyl-d-alanine ligase cell wall biosynthesis enzymes

    Inducible resistance to the glycopeptide antibiotics vancomycin and teicoplanin is mediated by plasmid pIP816 in Enterococcus faecium strain BM4147. Vancomycin induced the synthesis of a ca. 40 kDa membrane-assoc...

    Sylvie Dutka-Malen, Catherine Mohnas, Michel Arthur in Molecular and General Genetics MGG (1990)

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    Article

    Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545

    The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae transposes in various gram-positive bacterial genera following self-transfer and in Escherichia coli after cloning. Analysis of the junction...

    Frédéric Caillaud, Patrice Courvalin in Molecular and General Genetics MGG (1987)

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    Article

    Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: Evolutionary relationships and transcriptional analysis of aphA-3 genes

    The nucleotide sequence of the kanamycin resistance determinant aphA-3 encoded by transposon Tn1545 from Streptococcus pneumoniae was determined and compared to those of plasmids pJH1 and pIP1433 from Streptococc...

    Frédéric Caillaud, Patrick Trieu-Cuot, Cécile Carlier in Molecular and General Genetics MGG (1987)

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    Article

    Tn1545: A conjugative shuttle transposon

    Tn1545, from Streptococcus pneumoniae BM4200, confers resistance to kanamycin (aphA-3), erythromycin (ermAM) and tetracycline (tetM). The 25.3 kb element is self-transferable to various Gram-positive bacterial ge...

    Patrice Courvalin, Cécile Carlier in Molecular and General Genetics MGG (1987)

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    Article

    Transposable multiple antibiotic resistance in Streptococcus pneumoniae

    A mobile genetic element, designated Tn1545, was detected in the chromosome of Streptococcus pneumoniae BM4200, a clinical isolate multiply resistant to antibiotics. The 25.3 kb element conferred resistance to ka...

    Patrice Courvalin, Cécile Carlier in Molecular and General Genetics MGG (1986)

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    Article

    DNA sequences specifying the transcription of the streptococcal kanamycin resistance gene in Escherichia coli and Bacillus subtilis

    The gene conferring resistance to kanamycin, aphA, and originating from the streptococcal plasmid pJH1 was inserted into a shuttle vector. Full expression of aphA was obtained in Escherichia coli and Bacillus sub...

    Patrick Trieu-Cuot, André Klier, Patrice Courvalin in Molecular and General Genetics MGG (1985)

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    Article

    Tn1525, a kanamycin R determinant flanked by two direct copies of IS15

    We have isolated plasmid pIP112 (IncI1) from Salmonella panama and characterized by restriction endonucleases analysis and by recombinant DNA techniques a transposable element designated Tn1525. This 4.44 kilobas...

    Agnès Labigne-Roussel, Sylvie Briaux-Gerbaud in Molecular and General Genetics MGG (1983)

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    Article

    IS15, a new insertion sequence widely spread in R plasmids of gram-negative bacteria

    We have shown that the IS15 element, first detected in Salmonella ordonez and previously designated IS1522 (Labigne-Roussel et al. 1981), could transpose, with an approximate frequency of 5x10-5, to various sites...

    Agnès Labigne-Roussel, Patrice Courvalin in Molecular and General Genetics MGG (1983)

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    Article

    Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez

    Salmonella ordonez strain BM2000 carries kanamycin (Km), ampicillin (Ap), spectinomycin (Sp), chloramphenicol (Cm), tetracyline (Tc), and sulfonamide (Su) resistance and production of colicin Ib (Cib). The Km an...

    Agnés Labigne-Roussel, Guy Gerbaud, Patrice Courvalin in Molecular and General Genetics MGG (1981)