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Article
Open AccessDevelopment and functional testing of a novel in vitro delayed scratch closure assay
As the development of chronic wound therapeutics continues to expand, the demand for advanced assay systems mimicking the inflammatory wound microenvironment in vivo increases. Currently, this is performed in ...
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Article
Open AccessAn effective device to enable consistent scratches for in vitro scratch assays
The in-vitro scratch assay is a useful method in wound healing research to assess cell migration. In this assay, a scratch is created in a confluent cell layer by mechanically removing cells through manual scr...
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Protocol
Scale-Up of Single Cell–Inoculated Suspension Cultures of Human Embryonic Stem Cells
We have developed a simple yet highly reproducible technique of upscaling single cell–inoculated human embryonic stem cells (hESCs) in suspension cultures, using defined, nonconditioned media. Mass expansion o...
