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  1. No Access

    Article

    Chlorogenic Acids Biosynthesis in Centella asiatica Cells Is not Stimulated by Salicylic Acid Manipulation

    Exogenous application of synthetic and natural elicitors of plant defence has been shown to result in mass production of secondary metabolites with nutraceuticals properties in cultured cells. In particular, s...

    E. N. Ncube, P. A. Steenkamp, N. E. Madala… in Applied Biochemistry and Biotechnology (2016)

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    Article

    Optimization of Pressurized Hot Water Extraction of Flavonoids from Momordica foetida Using UHPLC-qTOF-MS and Multivariate Chemometric Approaches

    Pressurized hot water extraction (PHWE) has been classified as a green extraction technology, which is used for the extraction of bioactive compounds from plants. However, very few optimization strategies for ...

    B. S. Khoza, I. A. Dubery, H.-A. Byth-Illing, P. A. Steenkamp… in Food Analytical Methods (2016)

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    Article

    Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid

    Previous studies suggest that only trans-isomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated ...

    M. I. Mhlongo, L. A. Piater, P. A. Steenkamp, N. E. Madala… in Biotechnology Letters (2015)

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    Article

    Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions

    Nicotiana tabacum cell suspensions, 2 g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing ...

    Ntakadzeni E. Madala, P. A. Steenkamp, L. A. Piater, I. A. Dubery in Biotechnology Letters (2012)

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    Article

    Simultaneous determination of toxic heavy metals in Metformin hydrochloride using reversed-phase high-performance liquid chromatography

    A rapid and sensitive reversed phase HPLC technique was developed for the determination of seven toxic heavy metals in biological matrices. Cd, Pb, Ni, Co, Bi, Cu and Hg could be detected and quantified at ppb...

    P. A. Steenkamp, P. P. Coetzee in Fresenius' Journal of Analytical Chemistry (1993)