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Article
Pancreatic duct-like cell line derived from pig embryonic stem cells: expression of uroplakin genes in pig pancreatic tissue
The isolation of a cell line, PICM-31D, with phenotypic characteristics like pancreatic duct cells is described. The PICM-31D cell line was derived from the previously described pig embryonic stem cell-derived...
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Article
Feeder-cell-independent culture of the pig embryonic stem cell-derived exocrine pancreatic cell line, PICM-31
The adaptation to feeder-independent growth of a pig embryonic stem cell-derived pancreatic cell line is described. The parental PICM-31 cell line, previously characterized as an exocrine pancreas cell line, w...
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Article
Establishment and characterization of feeder cell-dependent bovine fetal liver cell lines
The establishment and initial characterization of bovine fetal liver cell lines are described. Bovine fetal hepatocytes were cultured from the liver of a 34-d bovine fetus by physical disruption of the liver t...
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Article
Proteome array identification of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses
Matrigel and similar commercial products are extracts of the Engelbreth-Holm–Swarm sarcoma that provide a basement-membrane-like attachment substrate or gel that is used to grow cells on or in, respectively. T...
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Article
A feeder-cell independent subpopulation of the PICM-19 pig liver stem cell line capable of long-term growth and extensive expansion
A method for the feeder-independent culture of PICM-19 pig liver stem cell line was recently devised, but the cell line’s growth was finite and the cells essentially ceased dividing after approximately 20 pass...
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Article
Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells
Feeder cells of irradiated mouse fibroblasts are commonly used for, and are generally necessary for, the in vitro maintenance and growth of many fastidious cell types, particularly embryonic stem cells or indu...
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Article
Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media
A serum-free, feeder cell-dependent, selective culture system for the long-term culture of porcine hepatocytes or cholangiocytes was developed. Liver cells were isolated from 1-wk-old pigs or young adult pigs ...
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Article
Feeder-independent continuous culture of the PICM-19 pig liver stem cell line
The PICM-19 pig liver stem cell line is a bipotent cell line, i.e., capable of forming either bile ductules or hepatocyte monolayers in vitro, that was derived from the primary culture of pig embryonic stem ce...
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Article
The effects of space flight and microgravity on the growth and differentiation of PICM-19 pig liver stem cells
The PICM-19 pig liver stem cell line was cultured in space for nearly 16 d on the STS-126 mission to assess the effects of spaceflight on the liver’s parenchymal cells—PICM-19 cells to differentiate into eithe...
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Article
Cytochrome P450 expression profile of the PICM-19H pig liver cell line: potential application to rapid liver toxicity assays
Liver in vitro models are needed to replace animal models for rapid assessment of drug biotransformation and toxicity. The PICM-19 pig liver stem cell line may fulfill this need since these cells have activiti...
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Article
The Pursuit of ES Cell Lines of Domesticated Ungulates
In contrast to differentiated cells, embryonic stem cells (ESC) maintain an undifferentiated state, have the ability to self-renew, and exhibit pluripotency, i.e., they can give rise to most if not all somatic...
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Article
Isolation and characterization of porcine visceral endoderm cell lines derived from in vivo 11-day blastocysts
Two porcine cell lines of yolk-sac visceral endoderm, designated as PE-1 and PE-2, were derived from in vivo 11-d porcine blastocysts that were either ovoid (PE-1) or at the early tubular stage of elongation (...
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Article
Establishment of a bovine blastocyst-derived cell line collection for the comparative analysis of embryos created in vivo and by in vitro fertilization, somatic cell nuclear transfer, or parthenogenetic activation
Tools and methods for analyzing differences in embryos resulting from somatic cell nuclear transfer (NT) in comparison to those derived from normal fertilization are needed to define better the nature of the n...
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Article
Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst
A cell line, BPE-1, was derived from a parthegogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously culture...
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Article
Macrophage population dynamics within fetal mouse fibroblast cultures derived from C57BL/6, CD-1, CF-1 mice and interleukin-6 and granulocyte colony stimulating factor knockout mice
In vitro models of macrophage growth, differentiation, and function are needed to facilitate the study of their biology as important immune facilitator cells and as frequent targets of bacterial and viral infe...
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Article
Spontaneous differentiation of porcine and bovine embryonic stem cells (Epiblast) into astrocytes or neurons
The culture of porcine or bovine epiblasts, i.e., embryonic stem cells, on STO feeder cells resulted in their spontaneous differentiation into multiple cell types that were subsequently isolated as separate ce...
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Article
Selective and organotypic culture of intrahepatic bile duct cells from adult pig liver
Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult...
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Article
Continuous culture of pig tissue-derived macrophages
Normal tissue macrophages from fetal and adult pig tissues can be continuously cultured by growing simple explant cultures on feeder layers of STO mouse embryonic fibroblasts. Macrophage cultures initiated fro...
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Article
Continuous cultures of macrophages derived from the 8-day epiblast of the pig
Secondary macrophage cell cultures were generated from the primary culture of epiblasts of 8-d-old pig blastocysts. The epiblast-derived macrophagelike (EDM) cells have a morphology and ameboid behavior that i...
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Article
A continuous culture of pluripotent fetal hepatocytes derived from the 8-day epiblast of the pig
Continuous cultures of pluripotent parenchymal hepatocytes were derived from the epiblasts of 8-day-old pig blastocysts. The cells were polygonal and had phase-contrast dark, granular cytoplasm with prominent ...
