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Article
Chromosome lesions in amniotic fluid cell cultures
The frequencies of chromosome lesions were determined on 3537 mitoses in samples of varying sizes from cultures of 25 amniotic fluid specimens taken from patients at cytogenetic risk. The average percentage va...
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Article
A duplication-deficiency X chromosome in a girl with severe mental retardation
Clinical and cytogenetic findings in a 14-year-old girl with severe mental retardation and partial Turner's syndrome are reported. An anomalous X was observed in the patient's karyotype. On the basis of QFQ. G...
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Chapter
Disappearance of Nuclear TdT in RPMI-8402 Following TPA Treatment
Terminal deoxynucleotidyl transferase (TdT) is a useful marker to study T-cell ontogeny and more generally lymphocyte differentiation.
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Chapter
Effect of Nucleosides on Human T and B Lymphoblastoid Cell Lines
Human T and B lymphoblastoid cell lines have been used as model systems to study the biochemical basis of some immune disfunctions, associated to specific purine metabolic enzyme deficiencies (1,2).
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Chapter
Clinical Relevance of Terminal Transferase and Adenosine Deaminase in Leukemia
Terminal Transferase (TdT), Adenosine Deaminase (ADA), immunological membrane markers, cytochemical reactivity and cytogenetics were analyzed in 226 patients with ALL, AUL and AML, in 70 patients with CML and ...
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Chapter
Adenosine Deaminase and Terminal Transferase in Leukemic Cells
The characterization of leukemic blasts has been recently greatly improved by the use of biochemical markers, such as Terminal deoxynucleotidyl Transferase (TdT) and Adenosine Deaminase (ADA).
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Chapter
The Mammalian ETS Genes: Two Unique Chromosomal Locations in Cat, Mice and Man and Novel Translocated Position in Human Leukemias
The ets sequence was identified as a second cellular sequence transduced by the avian replication-defective retrovirus, E26. The 5.7-kilobase (kb) RNA genome of E26 contains: in addition to partial retroviral gag
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Chapter and Conference Paper
Significance of Lineage-Associated Transcripts of TdT and ETS1 for Detection of Leukemic Cells
One approach for a rational postremission therapy of acute leukemia is based on detection of residual leukemic blasts. Immunophenoty** with monoclonal antibodies, quantitative DNA analysis for aneuploidy, PC...
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Article
Clonality analysis of hematopoietic cell lineages in acute myeloid leukemia and translocation (8;21): only myeloid cells are part of the malignant clone
Bone marrow from six patients with acute myeloid leukemia (AML) and t(8;21) (q22;q22) or a variant t(8;13;21) was studied by simultaneous analysis of cell morphology and karyotype. Combination of May–Grünwald–...
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Article
Bone marrow transplantation for chronic myeloid leukemia (CML) from unrelated and sibling donors: single center experience
This is a report on 60 consecutive patients with chronic myeloid leukemia (CML) who received an allogeneic bone marrow transplant (BMT) in this Unit. Donors were HLA-identical siblings (SIB) (n = 36) or unrelated...
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Article
Development of an interphase fluorescent in situ hybridization (FISH) test to detect t(8;21) in AML patients
The translocation (8;21) is a chromosome abnormality associated with acute myeloid leukemia (AML). As a consequence of the translocation the AML1(CBFA2) gene in the 21q22 region is fused to the ETO(CDR,MTG8) gene...
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Article
Subcellular localization of the oncoprotein MTG8 (CDR/ETO) in neural cells
The t(8;21) translocation associated with acute myeloid leukemia (AML) disrupts two genes, the AML1 gene also known as the core binding factor A2 (CBFA2) on chromosome 21, and a gene on chromosome 8, hereafter re...
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Article
Alternative donor transplants for patients with advanced hematologic malignancies, conditioned with thiotepa, cyclophosphamide and antithymocyte globulin
Preparative regimens without total body irradiation (TBI) have been reported for alternative donor hemopoietic stem cell transplants (HSCT). Between 7 September 1994 and 7 June 1999 48 patients with advanced h...
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Article
Improvement over time in outcome for children with acute lymphoblastic leukemia in second remission given hematopoietic stem cell transplantation from unrelated donors
Aims of this study were to verify whether reduction in transplant-related mortality (TRM) of children with acute lymphoblastic leukemia (ALL) in second complete remission (CR) given allogeneic hematopoietic st...
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Article
Impact of marrow unrelated donor search duration on outcome of children with acute lymphoblastic leukemia in second remission
We analyzed the outcome of 167 consecutive children with second CR acute lymphoblastic leukemia (ALL), for whom an unrelated donor (UD) search was activated between 1989 and 1998 at a median time of 2 months a...
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Article
RAR-mediated epigenetic control of the cytochrome P450 Cyp26a1 in embryocarcinoma cells
Retinoic acid (RA) is a signaling molecule that plays a pivotal role in major cellular processes and vertebrate development. RA action is mediated by specialized transcription factors, the nuclear RA receptors...
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Article
Progenitor cells trapped in marrow filters can reduce GvHD and transplant mortality
A bone marrow harvest is filtered either in the operating room, in the laboratory or during infusion to the patient. Filters are usually discarded. Little is known of haemopoietic progenitor cells (HPCs) trapp...
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Article
Allogeneic hemopoietic stem cell transplants for patients with relapsed acute leukemia: long-term outcome
We assessed the long-term outcome of patients with relapsed acute myeloid (n=86) or acute lymphoid leukemia (n=66), undergoing an allogeneic hemopoietic stem cell transplantation in our unit. The median blast cou...
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Article
Giuseppe Bigi (1944–2002)
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Article
Strategies of the donor search for children with second CR ALL lacking a matched sibling donor
During the last 10 years, the number of alternative Haematopoietic stem cell transplantations (HSCTs) performed on children in Europe has increased significantly and has reached 61% of the allografts. In this ...
