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Article
Open AccessThe sphingosine 1-phosphate receptor 2/4 antagonist JTE-013 elicits off-target effects on sphingolipid metabolism
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents...
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Article
Open AccessCytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma
While the two mammalian sphingosine kinases, SK1 and SK2, both catalyze the generation of pro-survival sphingosine 1-phosphate (S1P), their roles vary dependent on their different subcellular localization. SK1...
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Protocol
An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity
Sphingosine kinases (SK) are the sole enzymes responsible for the production of sphingosine 1-phosphate (S1P). S1P is a signaling molecule with a plethora of targets, acting as both a second messenger intracel...
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Protocol
Isoform-Selective Assays for Sphingosine Kinase Activity
Sphingosine kinases (SK) 1 and 2 are unique lipid kinases that phosphorylate sphingosine to form sphingosine-1-phosphate (S1P). S1P is a bioactive molecule eliciting multiple effects both extracellularly via ...
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Chapter and Conference Paper
Prediction of Dipeptidyl Peptidase (DP) 8 Structure by Homology Modelling
The DP8 model produced was very similar to the DPIV structure in the α/β hydrolase domain. Analysis of the active site of the DP8 model revealed significant structural conservation in the catalytic triad between ...
