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Sphingosine-1-Phosphate

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Alice Pébay

    1. University of Melbourne, Parkville, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Kursad Turksen

    1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Features the important effects of S1P in cell development and physiopathology
  • Includes detailed, tried-and-true protocols, ready for the lab
  • Contains expert tips and key troubleshooting advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 874)

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About this book

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field.

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate

    • Leyre Brizuela, Olivier Cuvillier
    Pages 1-20

  3. Isoform-Selective Assays for Sphingosine Kinase Activity

    • Melissa R. Pitman, Duyen H. Pham, Stuart M. Pitson
    Pages 21-31

  4. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry

    • Constantin Bode, Markus H. Gräler
    Pages 33-44

  5. A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule

    • Jun-ichi Morishige, Tamotsu Tanaka, Kiyoshi Satouchi
    Pages 45-54

  6. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples

    • Barbara Visentin, Gary Reynolds, Roger Sabbadini
    Pages 55-67

  7. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation

    • Hideru Obinata, Timothy Hla
    Pages 69-76

  8. High-Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes

    • William J. Valentine, Gabor Tigyi
    Pages 77-87

  9. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases

    • Matt D. Medlin, Joan M. Taylor, Christopher P. Mack
    Pages 89-97

  10. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm

    • Silvia A. Belmonte, Laila Suhaiman
    Pages 99-128

  11. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis

    • Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, Masaru Ishii
    Pages 129-139

  12. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System

    • Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, Timothy E. Kennedy
    Pages 141-154

  13. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells

    • Elisabetta Meacci, Francesca Bini, Chiara Battistini
    Pages 155-165

  14. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor

    • Raymond C. B. Wong, Martin F. Pera, Alice Pébay
    Pages 167-175

  15. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation

    • Erhard Bieberich
    Pages 177-192

  16. Sphingosine-1-Phosphate Signaling in Neural Progenitors

    • Phillip Callihan, Shelley B. Hooks
    Pages 193-200

  17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis

    • Shih-Chi Su, Kayla J. Bayless
    Pages 201-213

  18. Back Matter

    Pages 215-221
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Editors and Affiliations

  • University of Melbourne, Parkville, Australia

    Alice Pébay

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Sphingosine-1-Phosphate

  • Book Subtitle: Methods and Protocols

  • Editors: Alice Pébay, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-800-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-799-6Published: 19 April 2012

  • Softcover ISBN: 978-1-4939-5963-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-800-9Published: 23 April 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 221

  • Number of Illustrations: 29 b/w illustrations, 10 illustrations in colour

  • Topics: Cell Biology, Cell Culture

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