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Article
Open AccessLysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes
Lysyl hydroxylase 2 (LH2) is a member of LH family that catalyzes the hydroxylation of lysine (Lys) residues on collagen, and this particular isozyme has been implicated in various diseases. While its function...
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Article
Open AccessCollagen molecular phenotypic switch between non-neoplastic and neoplastic canine mammary tissues
In spite of major advances over the past several decades in diagnosis and treatment, breast cancer remains a global cause of morbidity and premature death for both human and veterinary patients. Due to multipl...
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Article
Open AccessA collagen glucosyltransferase drives lung adenocarcinoma progression in mice
Cancer cells are a major source of enzymes that modify collagen to create a stiff, fibrotic tumor stroma. High collagen lysyl hydroxylase 2 (LH2) expression promotes metastasis and is correlated with shorter s...
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Protocol
Glycosylation of Type I Collagen
Fibrillar type I collagen is the most abundant structural protein in most tissues and organs. One of the unique and functionally important characteristics of collagen is sequential posttranslational modificati...
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Protocol
Lysine Hydroxylation and Cross-Linking of Collagen
Collagens represent a large family of structurally related proteins containing a unique triple-helical structure. Among them, the fibril-forming collagens are the most abundant in vertebrates providing tissues...
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Article
Open AccessAuthor Correction: Pro-metastatic collagen lysyl hydroxylase dimer assemblies stabilized by Fe2+-binding
In the originally published version of this Article, financial support was not fully acknowledged. The PDF and HTML versions of the Article have now been corrected to also include support from the National Ins...
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Article
Open AccessIdentification of the effector domain of biglycan that facilitates BMP-2 osteogenic function
We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the spec...
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Article
Open AccessPro-metastatic collagen lysyl hydroxylase dimer assemblies stabilized by Fe2+-binding
Collagen lysyl hydroxylases (LH1-3) are Fe2+- and 2-oxoglutarate (2-OG)-dependent oxygenases that maintain extracellular matrix homeostasis. High LH2 levels cause stable collagen cross-link accumulations that pro...
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Article
Open AccessFKBP65-dependent peptidyl-prolyl isomerase activity potentiates the lysyl hydroxylase 2-driven collagen cross-link switch
Bruck Syndrome is a connective tissue disease associated with inactivating mutations in lysyl hydroxylase 2 (LH2/PLOD2) or FK506 binding protein 65 (FKBP65/FKBP10). However, the functional relationship between...
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Article
Open AccessDeletion of BMP receptor type IB decreased bone mass in association with compromised osteoblastic differentiation of bone marrow mesenchymal progenitors
We previously found that disruption of two type I BMP receptors, Bmpr1a and Acvr1, respectively, in an osteoblast-specific manner, increased bone mass in mice. BMPR1B, another BMP type I receptor, is also capable...
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Article
Unusual Fragmentation Pathways in Collagen Glycopeptides
Collagens are the most abundant glycoproteins in the body. One characteristic of this protein family is that the amino acid sequence consists of repeats of three amino acids –(X—Y—Gly)n. Within this motif, the Y ...
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Article
Simulation of postoperative 3D facial morphology using a physics-based head model
We propose a prototype of a facial surgery simulation system for surgical planning and the prediction of facial deformation. We use a physics-based human head model. Our head model has a 3D hierarchical struct...
