41 Result(s)

Article

Filamentous fungus-produced human monoclonal antibody provides protection against SARS-CoV-2 in hamster and non-human primate models

Monoclonal antibodies are an increasingly important tool for prophylaxis and treatment of acute virus infections like SARS-CoV-2 infection. However, their use is often restricted due to the time required for d...

Franziska K. Kaiser, Mariana Gonzalez Hernandez, Nadine Krüger… in Nature Communications (2024)

Article

Modification of transcriptional factor ACE3 enhances protein production in Trichoderma reesei in the absence of cellulase gene inducer

Trichoderma reesei is one of the best-known cellulolytic organisms, producing large quantities of a complete set of extracellular cellulases and hemicellulases for the degradation of lignocellulosic substances. H...

Yun Luo, Mari Valkonen, Raymond E. Jackson… in Biotechnology for Biofuels (2020)

Article

Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved ove...

Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota… in Biotechnology for Biofuels (2017)

Article

Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins a...

Christopher P. Landowski, Eero Mustalahti, Ramon Wahl… in Microbial Cell Factories (2016)

Article

The effects of extracellular pH and of the transcriptional regulator PACI on the transcriptome of Trichoderma reesei

Extracellular pH is one of the several environmental factors affecting protein production by filamentous fungi. Regulatory mechanisms ensure that extracellular enzymes are produced under pH-conditions in which...

Mari Häkkinen, Dhinakaran Sivasiddarthan, Nina Aro… in Microbial Cell Factories (2015)

Article

Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei

Trichoderma reesei is known as a good producer of industrial proteins but has hitherto been less successful in the production of therapeutic proteins. In order to elucidate the bottlenecks of heterologous protein...

Wesley Smith, Jussi Jäntti, Merja Oja, Markku Saloheimo in BMC Biotechnology (2014)

Article

Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase production

The soft rot ascomycetal fungus Trichoderma reesei is utilized for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. T. reesei uses several different enzymes for the degradat...

Mari Häkkinen, Mari J Valkonen, Ann Westerholm-Parvinen… in Biotechnology for Biofuels (2014)

Article

Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates

Trichoderma reesei is a soft rot Ascomycota fungus utilised for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. About 30 carbohydrate active enzymes (CAZymes) of T. reesei ...

Mari Häkkinen, Mikko Arvas, Merja Oja, Nina Aro, Merja Penttilä… in Microbial Cell Factories (2012)

Article

Correlation of gene expression and protein production rate - a system wide study

Growth rate is a major determinant of intracellular function. However its effects can only be properly dissected with technically demanding chemostat cultivations in which it can be controlled. Recent work on Sac...

Mikko Arvas, Tiina Pakula, Bart Smit, Jari Rautio, Heini Koivistoinen… in BMC Genomics (2011)

Article

The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30

Cellulase and hemicellulase genes in the fungus Trichoderma reesei are repressed by glucose and induced by lactose. Regulation of the cellulase genes is mediated by the repressor CRE1 and the activator XYR1. T. r...

M Carmen Limón, Tiina Pakula, Markku Saloheimo, Merja Penttilä in Microbial Cell Factories (2011)

Article

Erratum to: Discovery of a new tyrosinase-like enzyme family lacking a C-terminally processed domain: production and characterization of an Aspergillus oryzae catechol oxidase

Chiara Gasparetti, Greta Faccio, Mikko Arvas… in Applied Microbiology and Biotechnology (2011)

Article

Exploring laccase-like multicopper oxidase genes from the ascomycete Trichoderma reesei: a functional, phylogenetic and evolutionary study

The diversity and function of ligninolytic genes in soil-inhabiting ascomycetes has not yet been elucidated, despite their possible role in plant litter decay processes. Among ascomycetes, Trichoderma reesei is a...

Anthony Levasseur, Markku Saloheimo, David Navarro, Martina Andberg… in BMC Biochemistry (2010)

Article

Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae

Sulfhydryl oxidases are flavin-dependent enzymes that catalyse the formation of de novo disulfide bonds from free thiol groups, with the reduction of molecular oxygen to hydrogen peroxide. Sulfhydryl oxidases ...

Greta Faccio, Kristiina Kruus, Johanna Buchert, Markku Saloheimo in BMC Biochemistry (2010)

Article

Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties

Trichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use...

Marika Vitikainen, Mikko Arvas, Tiina Pakula, Merja Oja, Merja Penttilä… in BMC Genomics (2010)

Article

¹³C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose

The filamentous fungus Trichoderma reesei is an important host organism for industrial enzyme production. It is adapted to nutrient poor environments where it is capable of producing large amounts of hydrolytic e...

Paula Jouhten, Esa Pitkänen, Tiina Pakula, Markku Saloheimo… in BMC Systems Biology (2009)