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Open AccessCorrelation of gene expression and protein production rate - a system wide study
Growth rate is a major determinant of intracellular function. However its effects can only be properly dissected with technically demanding chemostat cultivations in which it can be controlled. Recent work on Sac...
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Article
The effect of temperature and pH gradients on Lactobacillus rhamnosus gene expression of stress-related genes
In this study, Lactobacillus rhamnosus, a renowned probiotic, was cultivated in fluctuating environment. Base gradients caused by a pH control in an industrial process and temperature gradients caused by uneven h...
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Article
Open AccessMonitoring of transcriptional regulation in Pichia pastoris under protein production conditions
It has become evident that host cells react to recombinant protein production with a variety of metabolic and intrinsic stresses such as the unfolded protein response (UPR) pathway. Additionally, environmental...
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Article
Open AccessGenome-wide analysis of protein production physiology in the filamentous fungus Trichoderma reesei
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Open AccessMonitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments
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Article
Open AccessSandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were...