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Article
Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed
The phototoxic red fluorescent GFP-like protein KillerRed has recently been described. The phototoxicity of KillerRed exceeds that of EGFP by at least 1,000-fold, making it the first fully genetically encoded ...
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Article
A genetically encoded photosensitizer
Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation1. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for...
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Article
Open AccessComplete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the biology and genetics of planaria, th...
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Article
Open AccessInterconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis
Within the family of green fluorescent protein (GFP) homologs, one can mark two main groups, specifically, fluorescent proteins (FPs) and non-fluorescent or chromoproteins (CPs). Structural background of diffe...