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Article
Changes in growth and oxidative response of leaves and nodules in Medicago ciliaris during salt stress recovery
In the present study, we exposed Medicago ciliaris isolated from a saline sebhka and growing symbiotically with Sinorhizobium medicae to 100 mM of NaCl and followed this stress by a recovery period (complete NaCl...
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Article
Open AccessThe biochemical composition and transcriptome of cotyledons from Brassica napus lines expressing the AtGL3 transcription factor and exhibiting reduced flea beetle feeding
Previously, transgenic trichome-bearing (hairy leaf) Brassica napus lines expressing either the Arabidopsis thaliana GL3 gene (line AtGL3+) [1] or the AtGL3 gene in combination with an RNAi construct to down-regu...
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Article
Assessment of salt tolerance of Nasturtium officinale R. Br. using physiological and biochemical parameters
Nasturtium officinale R. Br. seedlings were treated with a range of NaCl concentrations (0, 50, 100 and 150 mM) for 21 days after seedling emergence. Physiological analysis based on growt...
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Article
An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis
A novel orthologue of ethylene response factor gene, MsERF11, was isolated from alfalfa in this study. It has an open reading frame of 807 bp, encoding a predicted polypeptide of 268 amino acids. Sequence similar...
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Article
Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity
Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized fro...
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Article
Physiological and molecular responses of two Arabidopsis accessions to calcium amendment and salt constraint
The Arabidopsis thaliana NOK2 accession displays salt tolerance compared to more commonly known A. thaliana accessions, such as Col-0, but the basis of this phenotypic feature is unknown. This work was focused on...
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Article
Open AccessInvolvement of Source-Sink Relationship and Hormonal Control in the Response of Medicago Ciliaris — Sinorhizobium Medicae Symbiosis to Salt Stress
In order to explore the relationship between leaf hormonal status and source-sink relations in the response of symbiotic nitrogen fixation (SNF) to salt stress, three major phytohormones (cytokinins, abscisic ...
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Article
Open AccessIsolation and functional characterization of a Medicago sativa L. gene, MsLEA3-1
A full-length cDNA of 1,728 nt, called MsLEA3-1, was cloned from alfalfa by rapid amplification of cDNA ends from an expressed sequence tag homologous to soybean pGmPM10 (accession No. AAA91965.1). MsLEA3-1, enco...
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Article
DIMINUTO 1 affects the lignin profile and secondary cell wall formation in Arabidopsis
Brassinosteroids (BRs) play a crucial role in plant growth and development and DIMINUTO 1 (DIM1), a protein involved in BR biosynthesis, was previously identified as a cell elongation factor in Arabidopsis thalia...
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Article
Phenolic content and antioxidant activity in two contrasting Medicago ciliaris lines cultivated under salt stress
The objective of this study was to determine more indepth physiological and antioxidant responses in two Medicago ciliaris lines (a salt-tolerant line TNC 1.8 and a salt-sensitive line TNC 11.9) with contrasting ...
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Article
Variability in the response of six genotypes of N2-fixing Medicago ciliaris to NaCl
Genotypic variability was assessed within six Medicago ciliaris genotypes growing symbiotically with Sinorhizobium medicae in order to identify physiological criteria (growth, ion content, and plant health) assoc...
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Article
Molecular characterization of Brassica napus NAC domain transcriptional activators induced in response to biotic and abiotic stress
Subtractive expressed sequence tag analysis and screening of cDNA libraries derived from Brassica napus leaves subjected to mechanical wounding, flea beetle feeding or cold temperatures revealed eight genes encod...