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Assessment of salt tolerance of Nasturtium officinale R. Br. using physiological and biochemical parameters

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Abstract

Nasturtium officinale R. Br. seedlings were treated with a range of NaCl concentrations (0, 50, 100 and 150 mM) for 21 days after seedling emergence. Physiological analysis based on growth and mineral nutrition, showed a substantial decrease in leaf dry matter with 150 mM NaCl treatment. The growth decrease was correlated with nutritional imbalance and a reduction in potassium accumulation and transport to the leaves. At the same time, we noted an increase in leaf sodium and chloride accumulation and transport. Salt tolerance of N. officinale under 100 mM NaCl was associated with osmotic adjustment via Na+ and Cl− and the maintenance of high K+/Na+ selectivity. Salt decreased carotenoid content more than chlorophylls and also disturbed membrane integrity by increasing malondialdehyde content and electrolyte leakage. At 150 mM NaCl, an increase in antioxidant enzyme-specific activities for superoxide dismutase, catalase and guaiacol peroxidase occurred in concert with a decrease in ascorbic acid, polyphenol, tannin and flavonoid content. These results indicate that N. officinale can maintain growth and natural antioxidant defense compounds such as, vitamin C, carotenoids, and polyphenols, when cultivated in 100 mM NaCl, but not at higher salt levels.

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Abbreviations

DW:

Dry weight

FW:

Fresh weight

D:

Day

Chl:

Chlorophylls

CAR:

Carotenoids

EL:

Electrolyte leakage

MDA:

Malondialdehyde

SOD:

Superoxide dismutase

CAT:

Catalase

POD:

Guaiacol peroxidase

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Acknowledgments

Authors would like to thank all who participated in the elaboration of this work, with chemicals, instruments or critical reading.

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Authors and Affiliations

  1. Physiologie et Biochimie de la Tolérance des Plantes aux Contraintes Abiotiques, Faculté des Sciences de Tunis, Campus Universitaire, 2090, Tunis, Tunisia

    Rym Kaddour, Emna Draoui, Olfa Baâtour, Hela Mahmoudi, Imen Tarchoun, Nawel Nasri & Mokhtar Lachaâl

  2. Saskatoon Research Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, S7N0X2, Canada

    Margaret Gruber

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  1. Rym Kaddour
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  2. Emna Draoui
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  3. Olfa Baâtour
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Correspondence to Rym Kaddour.

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Communicated by J. Kovacik.

R. Kaddour and E. Draoui have equally participated in the elaboration of the manuscript.

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Kaddour, R., Draoui, E., Baâtour, O. et al. Assessment of salt tolerance of Nasturtium officinale R. Br. using physiological and biochemical parameters. Acta Physiol Plant 35, 3427–3436 (2013). https://doi.org/10.1007/s11738-013-1377-8

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