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Protocol
Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD+/NADH-Specific Fluorescent Protein Sensors
The pyridine nucleotides NAD(H) and NADP(H) are key molecules in cellular metabolism, and measuring their levels and oxidation states with spatiotemporal precision is of great value in biomedical research. Tra...
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Protocol
Identification of Peroxisome-Derived Hydrogen Peroxide-Sensitive Target Proteins Using a YAP1C-Based Genetic Probe
As the reversible oxidation of protein cysteine thiols is an important mechanism in signal transduction, it is essential to have access to experimental approaches that allow for spatiotemporal indexing of the ...
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Chapter
Peroxisomal Dysfunction and Oxidative Stress in Neurodegenerative Disease: A Bidirectional Crosstalk
Peroxisomes are multifunctional organelles best known for their role in cellular lipid and hydrogen peroxide metabolism. In this chapter, we review and discuss the diverse functions of this organelle in brain ...
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Article
Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina
The retinal pathology in peroxisomal disorders suggests that peroxisomes are important to maintain retinal homeostasis and function. These ubiquitous cell organelles are mainly involved in lipid metabolism, wh...
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Chapter
Peroxisomes and Cellular Oxidant/Antioxidant Balance: Protein Redox Modifications and Impact on Inter-organelle Communication
Disturbances in cellular redox balance have been associated with pro-aging mechanisms and increased risk for various chronic disease states. Multiple lines of evidence indicate that peroxisomes are central pla...
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Protocol
Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes
To gain additional insight into how specific cell organelles may participate in redox signaling, it is essential to have access to tools and methodologies that are suitable to monitor spatiotemporal difference...
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Protocol
KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress
Many biological processes and cell fate decisions are modulated by changes in redox environment. To gain insight into how subcellular compartmentalization of reactive oxygen species (ROS) formation contributes...
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Protocol
HaloTag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
Peroxisomes are multifunctional organelles that can rapidly modulate their morphology, number, and function in response to changing environmental stimuli. Defects in any of these processes can lead to organell...
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Chapter
Dissecting Peroxisome-Mediated Signaling Pathways: A New and Exciting Research Field
Peroxisomes are multifunctional organelles that play an important role in the metabolism of lipids and reactive oxygen species. As many cellular signaling functions are regulated via lipids, lipid second messe...
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Chapter
Aging, Age-Related Diseases and Peroxisomes
Human aging is considered as one of the biggest risk factors for the development of multiple diseases such as cancer, type-2 diabetes, and neurodegeneration. In addition, it is widely accepted that these age-r...
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Article
Peroxisomes in zebrafish: distribution pattern and knockdown studies
Peroxisomes are organelles that are essential for normal development in men and mice. In order to explore whether zebrafish could be used as a model system to study the role of peroxisomes, we examined their d...
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Article
Open AccessSmall G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6
Peroxisomes execute diverse and vital functions in virtually every eukaryote. New peroxisomes form by budding from pre-existing organelles or de novo by vesiculation of the ER. It has been suggested that ADP-ribo...
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Article
A mouse model for Zellweger syndrome
The cerebro-hepato-renal syndrome of Zellweger is a fatal inherited disease caused by deficient import of peroxisomal matrix proteins. The pathogenic mechanisms leading to extreme hypotonia, severe mental reta...
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Article
Metabolism of germinating teliospores of Ustilago nuda
Teliospores of Ustilago nuda are exogenously dormant. Germination and respiration of these thick-walled spores were greatly stimulated by glucose. Cycloheximide, actinomycine D, salicylhydroxamic acid and cyanide...
