Plasmodesmata
Methods and Protocols
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Living Reference Work Entry In depth
Typische Abdichtungsschäden aufgrund falscher Detailplanung
Damit sich der Ausspruch Planung ersetzt den Zufall durch den Irrtum nicht in Form eines Bauschadens oder eines bautechnischen Fehlers (Rechtsbegriff Mangel) manifestiert, braucht es weit mehr als nur die Beachtu...
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Protocol
In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata
The deposition and turnover of callose (beta-1,3 glucan polymer) in the cell wall surrounding the neck regions of plasmodesmata (PD) controls the cell-to-cell diffusion rate of molecules and, therefore, plays ...
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Book
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Protocol
In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG
Cells have developed mechanisms for cytoplasmic RNA transport and localization that participate in the regulation and subcellular localization of protein synthesis. In addition, plants can exchange RNA molecul...
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Protocol
Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses
Plasmodesmata (PD) are gated plant cell wall channels that allow the trafficking of molecules between cells and play important roles during plant development and in the orchestration of cellular and systemic s...
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Protocol
Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability
Plant virus movement proteins (MPs) mediate cell-to-cell movement of the virus genome through plasmodesmata (PD). MPs target PD to increase their size exclusion limit (SEL), and this MP function is essential f...
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Article
Open AccessTurnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes
Virus-induced plant diseases in cultivated plants cause important damages in yield. Although the mechanisms of virus infection are intensely studied at the cell biology level, only little is known about the mo...
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Book
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Protocol
Visualization of Transiently Expressed mRNA in Plants Using MS2
RNA transport and localization are evolutionarily conserved processes that allow protein translation to occur at specific subcellular sites and thereby having fundamental roles in the determination of cell fat...
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Article
Crosstalk between PTGS and TGS pathways in natural antiviral immunity and disease recovery
Virus-induced diseases cause severe damage to cultivated plants, resulting in crop losses. Certain plant–virus interactions allow disease recovery at later stages of infection and have the potential to reveal ...
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Article
Open AccessBioinformatic and expression analysis of the Brassica napus L. cyclophilins
Cyclophilins (CYPs) are a group of ubiquitous proteins characterized by their ability to bind to the immunosuppressive drug cyclosporin A. The CYP family occurs in a wide range of organisms and contains a cons...
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Chapter
Viral Transport and Interaction with the Host Cytoskeleton
Viruses depend on host cell functions for replication and for spreading their genomes between cells and throughout the organism. The spread of viruses in plants relies on the ability of virus-encoded movement ...
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Article
Open AccessThe Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA
The spread of Tomato yellow leaf curl virus (TYLCV) was accompanied by the formation of coat protein (CP) aggregates of increasing size in the cytoplasm and nucleus of infected tomato (Solanum lycopersicum) cells...
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Book
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Protocol
In Vivo RNA Labeling Using MS2
The trafficking and asymmetric distribution of cytoplasmic RNA is a fundamental process during development and signaling across phyla. Plants support the intercellular trafficking of RNA molecules such as gene...
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Protocol
Plasmodesmata: Channels for Viruses on the Move
The symplastic communication network established by plasmodesmata (PD) and connected phloem provides an essential pathway for spatiotemporal intercellular signaling in plant development but is also exploited b...
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Chapter
Viral Studies Point the Way: Mechanisms of Intercellular Transport
Plant development depends on the intercellular transport of macromolecules through gatable channels in the cell wall known as plasmodesmata (PD). Plant viruses exploit this intercellular trafficking pathway to...
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Article
Special issue: Plasmodesmata
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Article
Cellular pathways for viral transport through plasmodesmata
Plant viruses use plasmodesmata (PD) to spread infection between cells and systemically. Dependent on viral species, movement through PD can occur in virion or non-virion form, and requires different mechanism...
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Article
Interaction of the Tobacco mosaic virus movement protein with microtubules during the cell cycle in tobacco BY-2 cells
Cell-to-cell movement of Tobacco mosaic virus (TMV) involves the interaction of virus-encoded 30-kDa movement protein (MP) with microtubules. In cells behind the infection front that accumulate high levels of MP,...
