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Article
Open AccessMale pseudohermaphroditism in a complex malformed calf born with an acardius amorphus cotwin—a case report
Male pseudohermaphroditism is a developmental anomaly wherein animals are genetically and gonadally male, but their internal and/or external genitalia resemble those of females. In cattle, pseudohermaphroditis...
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Article
Open AccessPlasmodium berghei Brca2 is required for normal development and differentiation in mice and mosquitoes
Malaria is a major global parasitic disease caused by species of the genus Plasmodium. Zygotes of Plasmodium spp. undergo meiosis and develop into tetraploid ookinetes, which differentiate into oocysts that under...
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Article
Open AccessRole of Plasmodium berghei ookinete surface and oocyst capsule protein, a novel oocyst capsule-associated protein, in ookinete motility
Plasmodium sp., which causes malaria, must first develop in mosquitoes before being transmitted. Upon ingesting infected blood, gametes form in the mosquito lumen, followed by fertilization and differentiation of...
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Article
Improvement in identification of pro-estrous mice by using a novel method of detecting vaginal mucous cells
Accurate identification of the murine estrous cycle using vaginal exfoliative cytology is the initial and crucial step for controlled reproduction of this species. However, it is generally difficult to discrim...
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Article
Open AccessResponse to iron overload in cultured hepatocytes
Iron is essential for a variety of physiological processes. Hepatic iron overload acts as a trigger for the progression of hepatic steatosis to nonalcoholic steatohepatitis and hepatocellular carcinoma. In the...
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Article
Open AccessA new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing
Several bacterial moonlighting proteins act as adhesion factors, which are important for bacterial colonization of the gastrointestinal (GI) tract. However, little is known about the adherence properties of mo...
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Article
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice
The human cell line HK-2 is most commonly used as a model of renal proximal tubular epithelial cells (PTECs) for various studies despite the absence or low expression of transporters characteristic of parental...
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Article
Open AccessPlasmodium berghei Cap93, a novel oocyst capsule-associated protein, plays a role in sporozoite development
Disruption of Plasmodium oocyst capsule protein (PbCap380), and an oocyst wall interior protein, circumsporozoite protein, results in sporozoites not being formed, despite the formation of oocysts, and prevents m...
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Article
Open AccessRegulation of hepcidin expression by inflammation-induced activin B
Activin B is induced in response to inflammation in the liver and enhances hepcidin expression, but the source of activin B and the molecular mechanism underlying hepcidin induction are not clear yet. Lipopoly...
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Article
Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle
A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (...
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Article
A novel sapelovirus-like virus isolation from wild boar
A novel sapelovirus-like virus was isolated from a wild boar (Sus scrofa). In this study, partial viral genomic nucleotide sequences were determined using the rapid determination system of viral nucleic acid sequ...
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Article
Genetic diversity and classification of the outer capsid glycoprotein VP7 of porcine group B rotaviruses
We determined the nucleotide sequences of the outer capsid glycoprotein (VP7) genes of 38 porcine group B rotaviruses (GBRs) from feces of pigs at 27 farms in Japan between 2000 and 2007. Substantial diversity...
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Article
Region at amino acids 164 to 303 of the rabies virus glycoprotein plays an important role in pathogenicity for adult mice
The authors have previously reported that the glycoprotein of the pathogenic Nishigahara strain of rabies virus is required to lethality for adult mice. A cluster region of amino acid substitutions exists at t...
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Article
99mTc-tetrofosmin uptake in bone metastases from breast cancer
99mTc-tetrofosmin myocardial imaging was performed in a 62-year-old woman who had undergone standard radical mastectomy for right breast cancer 6 years ago. Although the result was negative for the ischemic heart...
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Article
Nucleotide sequence of the nucleoprotein gene of the RC·HL strain of rabies virus, a seed strain used for animal vaccine production in Japan
By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC·HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA cl...
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Article
Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein
The full-length cDNA corresponding to the mRNA encoding the nucleocapsid protein (NP) of rinderpest virus (RV) was cloned and its complete nucleotide sequence was determined. The gene of RV-NP was composed of ...
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Article
Receptor-mediated model relating anticonvulsant effect to brain levels of camazepam in the presence of its active metabolites
In a displacement test using3H-diazepam as a radioligand, the in vitro affinities of metabolites of camazepam (CZ) for the benzodiazepine receptors were 1–50 times more potent than that of CZ. In contrast, only t...
