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    Chapter

    Human Monoclonal Antibodies Produced by Epstein-Barr Virus Immortalized Cell Lines: Technical and Theoretical Principles

    Continuous cell lines that produce monoclonal antibodies are now routinely established from mouse and rat origin according to the hybridoma method (Köhler and Milstein, 1975). This method is still, to a large ...

    M. Steinitz in Reviews on Immunoassay Technology (1988)

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    Chapter and Conference Paper

    Establishment of Specific Antibody Producing Human Lines by Antigen Preselection and EBV-Transformation

    Human lymphocytes can be immortalized (“transformed”) by laboratory (B95-8) isolates of Epstein Barr virus (EBV) (1). Following primary infection, the virus induces EBNA, the EBV-determined nuclear antigen, in...

    M. Steinitz, S. Koskimies, G. Klein, O. Mäkelä in Lymphocyte Hybridomas (1979)