Abstract
Human lymphocytes can be immortalized (“transformed”) by laboratory (B95-8) isolates of Epstein Barr virus (EBV) (1). Following primary infection, the virus induces EBNA, the EBV-determined nuclear antigen, in B-lymphocytes only. Immortalized cell lines emerge in 10–20 days. Such lines, designated as LCL (lymphoblastoid cell lines) carry multiple copies of the viral genome and regularly express EBNA in 100% of the cells (2).
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References
Miller, G., Lipman, M.: Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc. Natl. Acad. Sci (USA) 70, 190–194 (1973).
Nilsson, K., Pontén, J.: Classification and biological nature of established human haematopoietic cell lines. Int. J. Cancer 15, 321–341 (1975).
Yefenof, E., Bakacs, T., Einhorn, L., Ernberg, I., Klein, G.: Epstein-Barr virus (EBV) receptors, complement receptor, and EBV infectibility of different lymphocyte fractions of human peripheral blood. I. Complement receptor distribution and complement binding by separated lymphocyte subpopulations. Cell. Immunol. 35, 34–42 (1978).
Einhorn, L., Steinitz, M., Yefenof, E., Ernberg, I., Bakacs, T., Klein, G.: Epstein Barr virus (EBV) receptors, complement and EBV-infectibility of different lymphocyte fractions of human peripheral blood. II. EBV studies. Cell. Immunol. 35, 43–58 (1978).
Steinitz, M., Bakács, T., Klein, G.: Interaction of the B95-8 and P3HR-1 substrains of Epstein Barr virus (EBV) with peripheral human lymphocytes. Submitted for publication (1978).
Steinitz, M., Klein, G., Koskimies, S., Mäkelä, O.: EB virus-induced B lymphocyte cell lines producing specific antibody. Nature 269, 420–422 (1977).
Böyum, A.: A one stage procedure for isolation of granulocytes and lymphocytes from human blood. Scand. J. Clin. Lab. Invest. 21, suppl. 97, 51–76 (1968).
Brownstone, A., Mitchison, N.A., Pitt-Rivers, R.: Chemical and serological, studies with an iodine containing synthetic immunological determinant 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) and related compounds. Immunology 10, 465–479 (1966).
Osoba, D.: Some physical and radiobiological properties of immunologically reactive mouse spleen cells. J. Exp. Med. 132, 368–383 (1970).
Steinitz, M., Klein, G.: Further studies on the differences in serum dependence in EBV negative lymphoma lines and their in vitro EBV converted, virus-genome carrying sublines. Europ. J. Cancer 13, 1269–1275 (1977).
Menezes, J., Leibold, W., Klein, G., Clements, G.: Establishment and characterization of an Epstein Barr virus (EBV) negative lymphoblastoid B cell line (BJAB) from an exceptional EBV genome negative African Barkitt’s lymphoma. Biomedicine 22, 276–284 (1975).
Klein, G., Giovanella, B., Westman, A., Stehlin, J.G., Mumford, D.: An EBV-genome-negative cell line established from an American Burkitt lymphoma; Receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5, 319–334 (1975).
Klein, E., Nilsson, K., Yefenof, E.: An establishedBurkitt’s lymphoma line with cell membrane IgG. Clin. Immunol. Immunopathol. 3, 575–583 (1975).
Cunningham, A.J., Szenberg, A.: Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology 14, 599–600 (1968).
Reedman, B.M., Klein, G.: Cellular localization of an Epstein Barr virus (EBV) associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int. J. Cancer 11, 499–520 (1973).
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Steinitz, M., Koskimies, S., Klein, G., Mäkelä, O. (1979). Establishment of Specific Antibody Producing Human Lines by Antigen Preselection and EBV-Transformation. In: Melchers, F., Potter, M., Warner, N.L. (eds) Lymphocyte Hybridomas. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67448-8_25
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DOI: https://doi.org/10.1007/978-3-642-67448-8_25
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