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Article
Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on ...
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Protocol
Generation and Evaluation of Bispecific Anti-TNF Antibodies Based on Single-Chain VHH Domains
Systemic cytokine inhibition may be an effective therapeutic strategy for several autoimmune diseases. However, recent studies suggest that tissue or cell type–specific targeting of certain cytokines, includin...
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Article
The Role of Microglia in the Homeostasis of the Central Nervous System and Neuroinflammation
Recently, much attention has been drawn to unraveling the mechanisms of neurodegenerative and neuroinflammatory disease pathogenesis. A special role in the development of neuropathologies is assigned to the in...
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Article
Proinflammatory Cytokines and Skin Wound Healing in Mice
Skin wound healing is subject to an intricate regulation, involves many cell populations and molecular mediators, and is one of the key mechanisms that ensures the barrier functions of the skin and the mainten...
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Article
Humanized Mouse Models as a Tool to Study Proinflammatory Cytokine Overexpression
Dysregulated proinflammatory cytokine expression may result in the development of severe pathologies, such as rheumatoid arthritis, psoriasis, and neurodegenerative diseases. Transgenic mice and, in particular...
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Article
Neonatal Lethality and Inflammatory Phenotype of the New Transgenic Mice with Overexpression of Human Interleukin-6 in Myeloid Cells
To model human interleukin-6 (hIL-6) associated diseases, unique mice with transgenic overexpression of human IL-6 and reporter fluorescent protein EGFP in cells of macrophage-monocyte lineage were generated u...
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Article
Proinflammatory and Immunoregulatory Functions of Interleukin 6 as Identified by Reverse Genetics
Reverse genetics approach, involving genome editing, makes it possible not only to establish the nonredundant and unique functions of genes and their products, but also to construct animal models for biomedica...
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Article
Cytokines as Mediators of Neuroinflammation in Experimental Autoimmune Encephalomyelitis
Cytokines play a pivotal role in maintaining homeostasis of the immune system and in regulation of the immune response. Cytokine dysregulation is often associated with development of various pathological condi...
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Article
Microbiota induces expression of tumor necrosis factor in postnatal mouse skin
Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the d...
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Article
Modeling of viral–bacterial coinfections at the molecular level using agonists of innate immunity receptors
The combined effect of innate immunity receptors in viral–bacterial coinfections was studied in vitro using the primary culture of murine macrophages activated by different combinations of ligands of innate im...
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Article
Myeloid-derived suppressor cells and proinflammatory cytokines as targets for cancer therapy
Myeloid-derived suppressor cells represent a heterogeneous population of immature myeloid cells. Under normal conditions, these cells differentiate into macrophages, dendritic cells, and granulocytes. However,...
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Article
Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice
The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interestin...
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Article
Mechanisms of changes in immune response during bacterial coinfections of the respiratory tract
Acute diseases of the respiratory tract are often caused by viral pathogens and accompanying secondary bacterial infections. It is known that the development of such bacterial complications is caused mainly by...
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Article
New Silk Fibroin-Based Bioresorbable Microcarriers
We fabricated bioresorbable microcarriers from water solution of Bombyx mori silk fi broin. The microcarriers are 3D structures with intricate surface and pores allowing penetration of culture medium, gas exchang...
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Article
Interleukin-6: From molecular mechanisms of signal transduction to physiological properties and therapeutic targeting
Interleukin-6 (IL-6) is one of the most important proinflammatory cytokines that has a broad spectrum of immunoregulatory properties. The molecular mechanisms of signal transduction of IL-6 and its receptor, w...
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Article
Novel 3D-microcarriers from recombinant spidroin for regenerative medicine
Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with ...
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Article
Formation of compact aggregates of B-lymphocytes in lung tissue during mycobacterial infection in mice depends on TNF production by these cells and is not an element of the host’s immunological protection
Tumor necrosis factor (TNF) plays a pivotal role in the early control of Mycobacterium tuberculosis and M. avium infections by a host. It was previously shown that both phagocyte-derived and T-cell-derived TNF pr...
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Article
Distinct biological activity of lipopolysaccharides with different lipid a acylation status from mutant strains of Yersinia pestis and some members of genus Psychrobacter
Correlation between the chemical structure of lipid A from various Gram-negative bacteria and biological activity of their lipopolysaccharide (LPS) as an agonist of the innate immune receptor Toll-like recepto...
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Article
Experimental models of arthritis in which pathogenesis is dependent on TNF expression
Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by joint damage as well as systemic manifestations. The exact cause of RA is not known. Both genetic and environmental factors are ...
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Article
Modern anti-cytokine therapy of autoimmune diseases
The emergence of genetically engineered biological agents opened new prospects in the treatment of autoimmune and inflammatory diseases. Cytokines responsible for regulation of a wide range of processes during...
